低聚果糖的遗传毒性研究

Study on genetic toxicity of the Fructo-oligosaccharide

目的研究低聚果糖致突变的可能性,探讨其是否具有遗传毒性。方法通过微核试验、精子畸形试验和沙门菌回复突变(Ames)试验,观察不同剂量的低聚果糖对小鼠骨髓细胞微核形成、精子畸形的产生和鼠伤寒沙门菌的回复突变情况的影响。结果 2.5 g/kg^10.0 g/kg BW低聚果糖处理的各剂量组小鼠的骨髓细胞微核形成率和精子畸形率都未出现明显增加,与相应的对照组相比无显著性差异;在加和不加代谢活化系统条件下,8μg/皿~5000μg/皿低聚果糖处理的各剂量组的鼠伤寒沙门菌回复突变率均未出现明显升高,与相应的对照组相比无显著性差异。结论在本研究条件下未发现低聚果糖具有直接或间接的致突变作用,也未发现其具有遗传毒性。

Objective To study the possibility of mutagenicity and the genetic toxicity of Fructo - oligosaccharide （FOS）. Methods Micronucleus test, sperm shape abnormality test and Ames test were employed to observe the effect of different doses of FOS on mouse bone marrow cells mieronucleus formation, sperm abnormalities and salmonella typhimurium reverse mutation. Results The rate of bone marrow cells micronueleus formation and sperm deformity rate showed no obvirous increase in mice treated with FOS between 2.5 g/kg BW - 10.0 g/kg BW, there was no significant difference from the corresponding control group ; With or without metabolic activation system, there was no significant increase in salmonella typhimurium reverse mutation rate with FOS dose between 8 μg/plate - 5000 μg/plate, and there was also no significant difference from the corresponding control group. Conclusion Based on this research, there was no direct or indirect mutagenic effect and genetic toxicity in Frueto - oligosaeeharide.