微波辅助提取葛根黄酮的工艺研究

Microwave-assisted extraction process of flavonoids from Radix puerariae

以葛根为原料,乙醇为溶剂,采用微波辅助提取葛根总黄酮。考察了溶剂体积分数、固液比、提取时间、微波功率以及提取次数对提取液葛根总黄酮含量的影响。并在单因素试验的基础上,进行4因素3水平的中心组合试验,通过响应面分析优化确定葛根总黄酮的提取工艺。结果表明,在乙醇体积分数56%、固液比1∶25、提取时间31s、微波功率539W、提取次数为2次的条件下总黄酮含量最高,模型预测值可达7.26mg/g,实际验证值为7.32mg/g。

The total flavonoids were extracted with ethanol as solvent from Radix puerariae by microwave-assisted extraction. The effects of ethanol volume fraction, solid to liquid ratio, extraction time, microwave power and extraction times on the flavonoid yield was investigated. On the basis of single factor experiments, a Box-Benhnken design involving four variables at three levels was employed, and the optimal values of these variables were determined by response surface analysis. The results showed that the optimum extraction conditions were as follows： ethanol volume fraction 56%, solid to liquid ratio 1：25, extraction time 31 s, microwave power 539 W and extraction times 2. Under this condition, the actually total flavonoid content was the highest with effective value was 7.32 mg/g, while the predicted value of the maximal yield of flavonoids was 7.26 mg/g.