低氧环境下去势大鼠骨质疏松模型的骨吸收标志物观察

Observation of Biochemical Indexes of Bone Resorption in Ovariectomized Rats with Osteoporosis in Hypoxia

观察低氧条件下去势大鼠骨质疏松模型的骨吸收相关生化指标变化,探讨其在低氧条件下骨质疏松症诊断和监测中的应用。选用纯种SD雌性大白鼠72只,随机分为A组(低氧去卵巢组)、B组(常氧去卵巢组)、C组(低氧对照组)和D组(常氧对照组)。术后分别在低氧模拟环境下和正常环境下饲养。并于术后4周、12周和24周测量大鼠的腰椎骨密度和相关生化指标,包括血浆抗酒石酸盐酸性磷酸酶、尿羟脯氨酸、尿脱氧吡啶酚。12周时,去卵巢大鼠骨密度下降较为显著(P<0.05),且低氧去卵巢组骨密度明显低于常氧去卵巢组(P<0.05),对照组均无显著变化(P>0.05)。24周,除常氧对照组骨密度无显著变化,其它三组骨密度均有显著降低,且低氧去卵巢组骨密度明显低于其它组,差异具有统计学意义(P<0.05)。骨吸收指标检测发现,12周时,所有组尿羟脯氨酸浓度无显著性差异(P>0.05),24周时,低氧去卵巢组血尿羟脯氨酸浓度显著高于其它三组(P<0.05)。12周时,去势大鼠的血浆抗酒石酸盐酸性磷酸酶和尿脱氧吡啶酚含量显著高于对照组,而饲养于低氧环境后,这种差异更加显著(P<0.01)。低氧环境可引起骨代谢异常,骨吸收增加,应用骨吸收生化指标检测有助于高空、高原低氧环境人群骨质疏松症的早期诊断。

To observe the changes of biochemical indexes of bone metabolism in ovariectomized rats with osteo- porosis in hypoxia and the application of biochemical indexes of bone resorption were explored in the diagnosis and monitoring of osteoporosis in hypoxia. 72 female SD rats were chosen and divided into group A（ ovariectomized group in hypoxia）, group B （ovariectomized group in normoxia）, group C （normal group in hypoxia） and group D（ normal group in normoxia）. After operation, the rats were bred in hypoxia and in normoxia respectively. The bone mineral density of the lumbar vertebrae and serum biochemical indexes were determined at 4,12,24 weeks. At 12 weeks after ovariectomy, the bone mineral density of ovariectomized rats was significantly decreased compared with the normal group （P 〈 0. 05 ）. The bone mineral density of ovariectomized group in hypoxia was lower than that of ones in normoxia（P 〈0. 05）. There were no obvious differences in the bone mineral density among the control groups （ P 〉 0.05 ）. Twenty-four weeks after operation , the bone mineral density of ovariectomized group in hypoxia was significantly lower than that in other groups（ P 〈0.05）. Detection of the biochemical indexes of bone metabolism： There were no obvious differences in urinary hydroxyproline in all groups at 12 weeks （ P 〉 0. 05 ）. Twenty-four weeks after operation ,the urinary hydroxyproline of ovariectomized group in hypoxia was significantly higherr than that in other groups （P 〈 0. 05 ）. The tartrate-resistant acid phosphatase and deoxypyridinoline in groups in hypoxia was significantly higher as compared with those in normoxia group（P 〈 0.01 ）. It suggested that the effect of oxygen deficit might cause abnormal bone metabolism and accelerate the bone resorption. The application of the biochemical indexes of bone resorption together with the bone mineral density is helpful for the diagnosis of osteoporosis in hypoxia.