基于Solexa高通量测序的香菇C_(91-3)功能基因的挖掘和开发

Exploration and development of functional genes from Lentinula edodes C_(91-3) based on Solexa high-throughput sequencing

香菇是全球人工种植最为普遍的食用菌,近年来,国内外的研究者对香菇进行了深入的研究,发现香菇在预防、治疗恶性肿瘤方面都具有重要的药用价值。尤其是香菇多糖制剂现已正式作为辅助化疗药物应用于临床。但是对香菇中蛋白质生物学活性的研究以及从蛋白质水平去发掘其抗肿瘤活性的功能基因却鲜有报道。本文拟就香菇的基因组、转录组及功能基因挖掘和开发现状进行简要论述。

Lentinula edodes is the most widely cultivated mushroom in the world. In recent years, domestic and foreign researchers found Lentinula edodes have an important medicinal value in the prevention, treatment of malignant tumors. As we all know, lentinan now is officially used as adjuvant chemotherapy drugs in clinic medicine. However, antitumor activity of Lentinula edodes was rarely reported at protein level, especially for functional genes. This review brief summarizes the advances in the genome, transcriptome and functional genomics of Lentinula edodes.