非小细胞肺癌患者肿瘤和癌旁组织p14ARF的甲基化状态检测及临床意义

The methylation status detected and analysis in clinic about p14ARF from non-small lung cancer tissues and its surrounding normal part

目的探讨非小细胞肺癌中的p14ARF基因启动子甲基化状态及其在非小细胞肺癌癌变中的作用。方法收集非小细胞肺癌及配对的癌旁组织各107例,采用改良巢式MSP(methylation specific PCR,MSP)检测肺癌患者肿瘤标本以及配对的癌旁组织中p14ARF基因外显子CpG岛的启动子甲基化情况。结果 p14ARF甲基化在107例肺癌中肿瘤组织中表达率为33.6%;而在配对的癌旁组织中表达率为12.1%;两组之间差异具有统计学意义(P<0.05),并且两者相关性极差。结论肺癌的癌组织及配对的癌旁组织中p14ARF启动子甲基化存在明显差异,结合其他辅助检查可提高肺癌诊断的阳性预测值。

Objective This study was designed to investigate promoter methylation status and protein expres-sion of p14ARF gene in non-small cell lung cancer and value the role of p14ARF promoter methylation in carcino-genesis of non-small cell lung cancer.Methods The study aimed to assess the methylation status of the CpG is-land of P14ARF promoter using modified NMSPCR （Nested methylation specific PCR）in the training set consisted of tumor tissues and paired para-carcinoma tissue in collecting 107 non-small lung cancer.Results The methyla-tion of p14ARF promoter was detected in 33.6% of tumor tissues,and 12.1% in distant-cancer mucosa respective-ly.The two results was a significant difference （P＆lt;0.05）,and the correlation of them is very poor.Conclusion The methylation status of p14ARF promoter exists a obvious difference in between tumor tissues and paired para-carcinoma tissues.So,it could improve PPV （positive predictive value）in clinic with other effective confirmed techniques.