PTEN与P-AKT在胃癌中的表达及意义

The expression of PTEN and P-AKT in gastric carcinoma and its significance

目的:检测人第10号染色体缺失的磷酸酶及张力蛋白同源基因(PTEN)与磷酸化丝氨酸/苏氨酸蛋白激酶B(P-AKT)在胃癌和癌旁组织中的表达,探讨它们在胃癌发生、发展中的作用。方法:应用免疫组织化学EliVision法,检测68例胃癌及其癌旁组织中PTEN和P-AKT蛋白表达水平,同时对68例胃癌和癌旁组织采用反转录-聚合酶链反应检测其中PTEN mRNA的表达。结果:PTEN蛋白在胃癌组织中表达低于癌旁组织(P<0.01),P-AKT蛋白在胃癌组织中表达高于癌旁组织(P<0.01),两者呈负相关关系(P<0.05)。胃癌组织中PTEN蛋白的表达在性别和年龄间差异均无统计学意义(P>0.05),但在分化程度、肿瘤大小、临床分期和淋巴结转移表达差异均有统计意义(P<0.01)。胃癌组织中P-AKT蛋白表达在性别、年龄和肿瘤大小间差异均无统计学意义(P>0.05),但在分化程度、临床分期和淋巴结转移间表达差异均有统计学意义(P<0.05)。胃癌组织PTEN mRNA的半定量表达明显低于癌旁组织PTEN mRNA的半定量表达(P<0.01)。结论:PTEN基因在胃癌组织中低表达,癌旁组织蛋白表达也明显高于胃癌组织,P-AKT蛋白在胃癌组织中的表达高于癌旁组织,两者在胃癌中的表达呈负相关。

Objective： To detect the expressions of phosphatase and tension protein homologous gene （PTEN） in human with chromosome 10 deletion and phosphorylated AKT（P-AKT） in gastric carcinoma and adjacent tissue, and explore their roles in the development of gastric carcinoma. Methods ：The mRNA and protein expression levels of I^FEN and P-AKT in 68 gastric carcinoma and adjacent gastric tissue specimens were detected using immunohistochemistry EliVision and reverse transcription polymerase chain reaction（ RT-PCR）, respectively. Results ：The PTEN and P-AKT protein levels in gastric carcinoma tissue specimen were significantly lower and higher than those in the adjacent tissue, respectively（ P 〈 0.01 ）, which was negative correlation（ P 〈 0.05 ）. No correlation between the expression of PTEN protein in gastric carcinoma and patients age and sexuality was found（ P 〉 0.05 ）, but the its expression was related to the differentiation degree, size, clinical stage and lymph node metastasis of tumor（ P 〈 0.01 ）. No correlation between the expression of P-AKT protein in gastric carcinoma and patients age and sexuality and tumor size was found （ P 〉 0.05 ）, but the its expression was related to the differentiation degree, clinical stage and lymph node metastasis of tumor （ P 〈 0.05 ）. The PTEN mRNA level in gastric carcinoma was significantly lower than that in adjacent tissue（P 〈 0.01 ）. Conclusions：The mRNA expression of PTEN gene in gastric carcinoma is lower,its protein expression in adjacent tissueis higher than that in gastric carcinoma. The expression of P- AKT protein in gastric carcinoma is higher than that in adjacent tissue. There is negatively correlated between the protein expressions of PTEN and P-AKT in gastric carcinoma.