毛细管电泳法分离分析乙酰半胱氨酸及四种相关杂质

Determination of acetycysteine and four relevant substances by CE

建立了分离分析乙酰半胱氨酸及4种相关杂质的毛细管电泳法。采用熔融石英毛细管（50 μm i.d.×50cm，有效长度为45cm），以y（50mmol／LNaH2PO4（pH7.0））：V（甲醇）=97：3为背景缓冲溶液，进样时间20S，运行电压15kV，检测波长210 nm。在优化的条件下，乙酰半胱氨酸与4种相关杂质在15min内均达到基线分离，乙酰半胱氨酸质量浓度在20～500μg／mL的范围内，具有良好的线性关系（r^2=0.9998），检出限为3 μg／mL（S／N=3），加标回收率为98.8％～102.0％，相对标准偏差为0.29％～0.91％。方法已用于实际样品的分析。

A capillary electrophoresis （CE） method was established for the separation and determination of acetycysteine and four relevant substances. The CE analysis was carried out using an uncoated capillary electrophoresis with 50 μm i.d. and 50 cm （45 cm to the detector） total length. The running buffer was 50 mmol/L sodium dihydrogen phosphate （ pH ： 7.0） ： methanol = 97 ： 3 with the sample injection of 20 s, a wavelength of 210 nm and a separation voltage of 15 kV. Under the optimized conditions, acetycysteine and four relevant substances were baseline separated within 15 minutes. The developed method had a good linearity in the range of 20 - 500 μg/mL with r^2 = 0.9998. The limit of detection was 3 μg,/mL （ S/N = 3 ）. The spike recoveries ranged from 98.8% to 102.0% with the acceptable The method is simple, sensitive, accurate and reliable for the relative standard deviations of 0.29% to 0.91%. determination of real samples.