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利用CLIP技术研究蛋白质和RNA的相互作用 被引量：3

CLIP techniques in studying protein-RNA interactions
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摘要 紫外交联免疫共沉淀(CLIP)技术能揭示RNA结合蛋白质在体内的RNA结合位点。将讨论该技术的基本原理和一些新的发展,这些新发展显著地提高了技术的特异性、灵敏度和应用范围。 Ultraviolet-crosslinking and immunoprecipitation （CLIP） techniques can provide key information about the in vivo RNA-binding site of protein. This review will discuss the basic principles and recent developments of CLIP techniques. These new developments have significantly improved the specificity, sensitivity and application range of the technique.
作者 周德建 叶克穷
机构地区 北京生命科学研究所 北京协和医学院研究生院 中国医学科学院
出处 《生命科学》 CSCD 2014年第3期207-213,共7页 Chinese Bulletin of Life Sciences
基金 国家重点基础研究发展计划("973"项目)(2010CB835400)
关键词 紫外交联 蛋白质RNA相互作用 深度测序 UV crosslinking protein-RNA interaction deep sequencing
分类号 Q-33 [生物学]
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参考文献15

  • 1Ule J, Jensen K, Mele A, et al. CLIP: a method for identifying protein-RNA interaction sites in living cells. Methods, 2005, 37(4): 376-86.
  • 2Ule J, Jensen KB, Ruggiu M, et al. CLIP identifies Nova- regulated RNA networks in the brain. Science, 2003, 302(5648): 1212-5.
  • 3Darnell RB. HITS-CLIP: panoramic views of protein- RNA regulation in living cells. Wiley Interdiscip Rev RNA, 2010, 1(2): 266-86.
  • 4Kudla G, Granneman S, Hahn D, et al. Cross-linking, ligation, and sequencing of hybrids reveals RNA-RNA interactions in yeast. Proc Natl Acad Sci USA, 2011, 108(24): 10010-5.
  • 5Hafner M, Landthaler M, Burger L, et al. Transcriptome- wide identification of RNA-binding protein and microRNA target sites by PAR-CLIP. Cell, 2010, 141(1): 129-41.
  • 6Granneman S, Kudla G, Petfalski E, et al. Identification of protein binding sites on U3 snoRNA and pre-rRNA by UV cross-linking and high-throughput analysis of cDNAs. Proc Natl Acad Sci USA, 2009, 106(24): 9613-8.
  • 7Helwak A, Kudla G, Dudnakova T, et al. Mapping the human miRNA interactome by CLASH reveals frequent noncanonical binding. Cell, 2013, 153(3): 654-65.
  • 8Darnell R. CLIP (cross-linking and immunoprecipitation) identification of RNAs bound by a specific protein. Cold Spring Harb Protoc, 2012, 2012(11): 1146-60.
  • 9Wang Z, Tollervey J, Briese M, et al. CLIP: construction of cDNA libraries for high-throughput sequencing from RNAs cross-linked to proteins in vivo. Methods, 2009, 48(3): 287-93.
  • 10Ascano M, Hafner M, Cekan P, et al. Identification of RNA-protein interaction networks using PAR-CLIP. Wiley Interdiscip Rev RNA, 2012, 3(2): 159-77.

同被引文献33

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  • 9Mercer TR, Qureshi IA, Gokhan S, et al. Long noneoding RNAs in neumnal-glial fate specification and oligodendmcyte lineage maturation [J]. BMC Neumsei,2010,11:14.
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生命科学
2014年 第3期

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