核糖体展示技术在非编码核酸研究中的应用

Ribosome profiling and its application in non-coding RNA study

非编码RNA(non-coding RNA)是一类内源性的不具有蛋白质编码功能的RNA分子,在细胞的生长增殖、发育、核内运输,甚至在肿瘤的发生中发挥着重要的作用。核糖体展示技术(ribosome profiling)是多聚核糖体分离和深度测序技术(deep sequencing)相结合的新兴组学技术,可以精确地测定正在被翻译的转录本并具有组学广度。对非编码RNA研究而言,这既是一个新的高度又是一门新的技术。系统阐述了核糖体展示技术的实验原理、目前研究取得的进展,以及在非编码RNA调控机理中的应用。

Non-coding RNA （ncRNA） is a class of endogenous RNA molecules playing important roles in diverse biological process, such as cell proliferation, development, nuclear transport, and tumorigenesis. Ribosome profiling is a novel technology combing polyribosome isolation and deep sequencing to determine the cellular transcripts being translated. In this review, we summarized the current state of art of ribosome profiling and discussed the potential application of ribosome profiling in ncRNA research.