毒理学研究中的体外细胞毒性评价

In vitro cytotoxicity evaluation in toxicology

体外细胞毒性评价作为传统动物模型毒性评价的替代方法正在得到越来越多的研究和应用,而其向高通量阶段的迈进则为新毒物和新药物的检测与目的物的筛选提供了更加快捷、高效的手段。将对体外细胞毒性评价常用细胞类型、体外细胞毒性评价的指标,及其检测技术方法的研究现状、进展和存在问题进行阐述,希望能为相关的研究提供一定的参考。

Abstract： In vitro cytotoxicity evaluation, as an alternative of traditional toxicity evaluation using animal models, has been widely studied and applied. The development of cell-based high-throughput detecting and screening assays will provide fast and efficient means for detecting new toxic substances and drugs and screening target ingredients. The current research status, scientific progress, and existing problems of commonly used cell types, different test indexes and methods for in vitro cytotoxicity assessments are reviewed, which will provide some reference for related research. Key words： toxicology; in vitro assay; cytotoxicity evaluation; high-throughput screening