牛大力多糖对小鼠抗疲劳作用的研究

In vivo Anti-fatigue Effect of Polysaccharides from Millettia speciosa Champ.

本文以牛大力(Millettia speciosa Champ.)为对象,利用小鼠爬杆和负重游泳为实验模型,研究了其多糖的抗疲劳作用。实验随机将小鼠分为五组,分别为空白对照组、阳性药(人参蜂王浆,7 mL/kg)对照组、牛大力多糖低剂量组[212.5 mg/(kg·d)]、中剂量组[425 mg/(kg·d)]、高剂量组[850 mg/(kg·d)],灌胃给药14 d后,考察其对小鼠爬杆时间、负重游泳时间以及血乳酸(LD)、血乳酸脱氢酶(LD-H)、血中尿素氮(BUN)含量的影响。结果表明,牛大力多糖能延长小鼠爬杆时间,增加小鼠游泳耐力,降低LD、BUN的含量、提高血中LD-H含量。且中剂量的药效与阳性药对照组的基本相当。

In this paper,the anti-fatigue effect of polysaccharides from Millettia speciosa Champ. （NDLs） was studied by models of mice pole-climbing and loaded swimming. Mice were grouped randomly to create five groups, including negative control group;positive control （ginseng reyal jelly,7 mL/kg） ;and polysaccharide groups at low, medium, and high doses [212.5,425,850 mg/（kg . d）]. All groups were treated by gavage for 14 consecutive days. The pole-climbing time,loaded swimming time, the values of blood lactic acid, blood lactate dehydrogenase-L, and blood urea nitrogen were determined. The results indicated that NDLs can significantly prolong the pole-climbing and loaded swimming time of mice, reduce the levels of blood lactic acid and blood urea nitrogen, and improve the content of blood lactate dehydrogen- ase-L when compared with the control group （ P 〈 0. 05）. Furthermore ,the effect of the medium dose group was similar to that of positive control group and was not improved obviously after the dose doubled. Based on the above results, it was concluded that NDLs have an excellent anti-fatigue effect and 425 mg/（kg . d） maybe the limit of gavage after consid- ered absorption effects of mice.