不同提取方法对印度紫檀叶片挥发性成分的GC-MS分析

Analysis of Volatile Components from Pterocarpus indicus Leaves with Different Extraction Methods by Gas Chromatography-Mass Spectrometry

采用气相色谱-质谱法(GC-MS)分析同时蒸馏萃取法(SDE)、固相微萃取法(SPME)和动态顶空吸附法(DHA)三种方法提取的印度紫檀挥发性成分。结果表明:三种方法提取印度紫檀挥发油的化学成分有差异。同时蒸馏萃取法提取印度紫檀挥发油鉴定出25种化合物,主要成分为2-硝基乙醇,相对含量为35.29%;固相微萃取法提取印度紫檀挥发油鉴定出27种化合物,主要成分为乙酸叶醇酯,相对含量为33.62%;动态顶空吸附法提取印度紫檀挥发油鉴定出27种化合物,主要成分为2-硝基乙醇,相对含量为30.42%。三种方法共检测到2种相同组分,检出的主要物质是醇类、酯类和缩醛。

The volatile components from Pterocarpus indicus leaves were extracted by simultaneous distillation extraction （SDE） ,solid phase micro-extraction （SPME） and dynamic headspace adsorption （DHA） and analyzed using gas chromatography-mass spectrometry （GC-MS）. The results indicated that the volatile oil compounds extracted out using the three methods were different. Twenty-five chemical components were identified from the SDE extract, the main component was 2-nitroethanol, accounting for 35.29% of the total identified components ;Twenty-seven chemical components were i- dentified from the SPME extract, the main component was cis-3-hexenyl-l-acetate, accounting for 33.62% of the total i- dentified components;Twenty-seven chemical components were identified from the DHA extract, the main component was 2-nitroethanol,accounting for 30.42% of the total identified components. Two compounds were detected in all the three extracts. In addition, alcohols, esters and acetals were the major components detected in all the three extracts.