几种天然有机多酚酸体外抗脂质过氧化研究

Anti-lipoperoxidation of Several Organic Polyphenol Acids in vitro

通过天然有机多酚酸对H2O2诱导小鼠肝匀浆脂质过氧化和自发脂质过氧化的保护作用的体外实验,比较研究绿原酸、咖啡酸、迷迭香酸、阿魏酸、丹参素、原儿茶醛和3-羟基肉桂酸体外抗氧化能力的强弱,探讨其抗氧化能力产生机理及构效关系。结果表明,7种多酚酸对H2O2诱导小鼠肝匀浆脂质过氧化的保护作用强弱依次为迷迭香酸>咖啡酸>原儿茶醛>绿原酸>阿魏酸>丹参素>3-羟基肉桂酸,其最大和最小抑制率相差2.4倍;对小鼠肝脏组织自发脂质过氧化的抑制率大小顺序为迷迭香酸、原儿茶醛、绿原酸、咖啡酸、阿魏酸、丹参素、3-羟基肉桂酸,最高和最低抑制率相差近90%。7种天然有机酸抗脂质过氧化作用因化合物结构不同存在显著差异。

The natural polyphenol acids are reported to possess strong antioxidative activity. In this study, the effects of seven polyphenol acids including rosmarinic acid, coffeic acid, protocatechuic aldehyde, chlorogenic acid, ferulic acid, salvianic acid and 3-hydroxycinnamic acid, against lipid peroxidation in vitro were investigated. The thiobarbituric acid （TBA） assay was used to evaluate the spontaneous and H2O2 induced lipid peroxidation in rat-hver homogenate. The results showed that all seven polyphenol acids possessed strong anti-lipid peroxidation activities. The order of their activities against H2O2 induced-lipld peroxidation was rosmarinie acid 〉 coffeie acid 〉 protocatechuic aldehyde 〉 chlorogenic acid 〉 fendic acid 〉 salvianic acid 〉 3-hydroxycinnamic acid. The order of their activities against spontaneous lipid peroxidation was msmarinic acid 〉 protocatechuic aldehyde 〉 chlorogenic acid 〉 coffeic acid 〉 ferulic acid 〉 salvianic acid 〉 3-hydroxycinnamic acid. The anti-lipid peroxidation of seven kinds of organic acid existed significant differences for their different structure.