枸杞多糖对脑缺血再灌注损伤模型大鼠的保护作用

Protective Effect of Lycium barbarum Polysaccharides on Cerebral Ischemia-reperfusion Injury Model Rats

目的:研究枸杞多糖对脑缺血再灌注损伤模型大鼠的保护作用。方法:采用线栓法复制大鼠脑缺血再灌注损伤模型。84只SD大鼠随机均分为正常对照(等容生理盐水)组、假手术(等容生理盐水)组、模型(等容生理盐水)组、尼莫地平(0.5 mg/kg)组与枸杞多糖高、中、低剂量(60.0、30.0、15.0 mg/kg)组。于复制模型前2 d开始灌胃给药,每天2次,连续3 d。对大鼠进行神经症状评分,测定大鼠脑含水量,逆转录-聚合酶链反应(RT-PCR)法测定半胱氨酸蛋白酶(Caspase)-12 mRNA的表达,Western blot法测定Caspase-12蛋白的表达。结果:与假手术组比较,模型组大鼠神经症状评分、脑含水量增加,Caspase-12 mRNA和蛋白表达增强,差异有统计学意义(P<0.01);与模型组比较,枸杞多糖高、中剂量组大鼠神经症状评分、脑含水量减少,Caspase-12 mRNA和蛋白表达减弱,差异有统计学意义(P<0.01或P<0.05)。结论:枸杞多糖对脑缺血再灌注损伤模型大鼠有一定保护作用,其机制与减少神经症状评分、脑含水量及调节Caspase-12 mRNA与蛋白的表达有关。

OBJECTIVE： To study the protective effect of Lycium barbarum polysaccharides on cerebral ischemia-reperfusion injury model rats. METHODS： Cerebral ischemia-reperfusion injury was induced by suture method. 84 SD rats were randomly di- vided into normal control group （constant volume of normal saline）, sham operation group （constant volume of normal saline）, model group （constant volume of normal saline）, nimodipine group （0.5 mg/kg）, L. barbarum polysaccharide high-dose, medi- um-dose, low-dose groups （60.0, 30.0, 15.0 mg/kg）. They were given relevant medicines intragastrically 2 days before modeling, twice a day, for consecutive 3 days. Neural symptoms score and the determination of water content in cerebral tissue were carried out. RT-PCR method was used to determine the expression of caspase-12 mRNA, and Western blot method was used to determine the expression of Caspase-12 protein. RESULTS： Compared with sham operation group, the neurological symptom score, brain wa- ter content, and the expression of caspase-12 mRNA and protein were all significantly increased; there was statistical significance （P〈0.01）. Compared with model group, neurological symptom score, brain water content, the expression of Caspase-12 mRNA and protein were all significantly decreased, there was statistical significance （P〈0.01, P〈0.05）. CONCLUSIONS： L. barbarum polysaccharide can protect the rat from cerebral ischemia-reperfusion injury, Which maybe associated with the reduction of neural symptoms score and brain water content, and the regulation of Caspase-12 mRNA and protein expression.