摘要
目的:研究七叶皂苷钠对急性肺损伤模型大鼠的保护作用。方法:45只SD大鼠随机均分为正常对照(等容生理盐水)组、模型(等容生理盐水)组、七叶皂苷钠(5 mg/kg)组。大鼠尾静脉缓慢注射油酸(0.1 ml/kg)以复制急性肺损伤模型,模型复制30 min后尾静脉给药。光镜下观察大鼠肺组织病理切片;称定大鼠肺湿质量、干质量,并计算肺湿/干质量比(W/D);ELISA法检测大鼠血清肿瘤坏死因子(TNF)-α含量;Western blot法检测大鼠肺组织水通道蛋白(AQP)1的表达。结果:与正常对照组比较,模型组大鼠肺组织中肺泡腔内炎症细胞渗出增加,W/D升高,TNF-α含量增加,AQP1蛋白表达增强,差异有统计学意义(P<0.05);与模型组比较,七叶皂苷钠组大鼠肺组织水肿程度明显改善,W/D降低,TNF-α含量减少,AQP1蛋白表达减弱,差异有统计学意义(P<0.05)。结论:七叶皂苷钠可减轻油酸致大鼠急性肺损伤程度,其机制可能与降低W/D、减少TNF-α含量、下调AQP1蛋白表达有关。
OBJECTIVE： To investigate protective effect of sodium aescinate on acute lung injury （ALl） model rats. METHODS： 45 SD rats were randomly divided into normal control group （constant volume of normal saline）, model group （constant volume of normal saline） and sodium aescinate group （5 mg/kg）. ALI model was made by intravenous injection of oleic acid （0.1 ml/ kg）. 30 min after modeling, they were given relevant medicines via tail vein. The histological section of lung tissue was observed under light microscope. The lung wet and dry weight were determined to calculate lung wet/dry weight ratio （W/D） ; TNF-ct concen- tration and expression of AQP1 were determined by ELISA assay and Western blot, respectively. RESULTS：Compared with normal control group, the effusion of inflammatory cell in the alveolar space increased in model group, while W/D, TNF-α concentration and protein expression of AQP1 increased; there was statistical significance （P〈 0.05）. Compared with model group, pneumonede- ma of rats was improved significantly in sodium aescinate group, while W/D, TNF-ct concentration and protein expression of AQP1 decreased; there was statistical significance（P〈0.05）. CONCLUSIONS： Sodium aescinate may alleviate oleic acid-induced ALI in rats, the mechanism of which may be associated with the down-regulation of W/D, TNF-α concentration and protein expression of AQP1.
出处
《中国药房》
CAS
CSCD
2014年第15期1368-1371,共4页
China Pharmacy
