RP-HPLC法同时测定人血浆中马钱子4种生物碱的含量

Establishment of RP-HPLC Method for the Content Determination of 4 Kinds of Alkaloids from Strychnos nux-vomica in Human Plasma

目的：建立同时测定人血浆中马钱子4种生物碱含量的方法。方法：采用反相高效液相色谱（RP．HPLC）法。色谱柱为HYP－0DSC18（250mm×4．6mm，5μm），流动相为乙腈．0．2％十二烷基硫酸钠溶液（40：60，V/V，pH2．8），流速为1．0ml／min，柱温为30℃，检测波长为254nm。结果：番木鳖碱、马钱子碱、番木鳖碱氮氧化物、马钱子碱氮氧化物的质量浓度分别在0．53-53．40、0．54～53．55、0．53-53．40、0．53-53．25μg／ml范围内与待测物峰面积积分值呈良好线性关系（r≥0．9980）；精密度的RsD〈9．0％，稳定性的RSD均在2．15％～7．69％之间；提取回收率分别为1．05％～2．36％、1．32％～2．43％、1．44％～2．55％和1．52％～2．69％。结论：该方法灵敏度高、精密度好、简便可行，可用于马钱子中4种生物碱的药动学研究。

OBJECTIVE： To establish the method for the content determination of 4 kinds of alkaloids from Strychnos nux-vomica in huamn plasma. METHODS： RP-HPLC method was adopted. The determination was performed on HYP-ODS C18（250 mm× 4.6 mm, 5 μm） column with mobile phase consisted of acetonitrile-0.2 % sodium lauryl sulfate （40：60, V/V, pH 2.8） at the flow rate of 1.0 ml/min. The colunm temperture was 30 ~C, and the detection wavelength was 254 mn. RESULTS： The linear ranges of strych- nine, brucine, strychnine N-oxide and brucine N-oxide were 0.53-53.40,0.54-53.55,0.53-53.40, 0.53-53.25 μg/ml（r≥0.998 0） ; RS- Ds of precision tests were less than 9.0% ; RSDs of stability tests were 2.15%-7.69% ; extraction recoveries were 1.05%-2.36%, 1.32%-2.43%, 1.44%-2.55% and 1.52%-2.69%, respectively. CONCLUSIONS： The method is sensitive, precisive, convenient and feasible, and can be used for pharmacokinetic study of 4 kinds of alkaloids from S. nux-vomica.