重庆秀山地区灰毡毛忍冬的HPLC指纹图谱研究

Study on HPLC Fingerprints of Lonicera macranthoides from Chongqing Xiushan Area

目的:建立重庆秀山地区灰毡毛忍冬药材的指纹图谱。方法:采用高效液相色谱(HPLC)法。色谱柱为Phenomenex Synergi Hydro-RP 80A C18(250 mm×4.6 mm,4μm),流动相为乙腈-0.5%磷酸水溶液(梯度洗脱),流速为1 ml/min,柱温为35℃,检测波长为327 nm。采用《中药色谱指纹图谱相似度评价系统》(2004A版)对样品进行相似度评价。结果:来自重庆秀山地区28个不同产地的灰毡毛忍冬药材的HPLC指纹图谱相似度较高(均大于0.96)、分离度较好,共标定出8个共有指纹峰,符合中药色谱指纹图谱研究的技术要求。结论:该方法准确、可靠,可为灰毡毛忍冬药材的质量控制提供科学依据。

OBJECTIVE： To establish the fingerprint of Lonicera macranthoides from Chongqing Xiushan area. METHODS： HPLC method was adopted. The separation was performed on Phenomenex Synergi Hydro-RP 80A C18（250 mm×4.6 mm, 4 μm） with mobile phase consisted of acetonitril-0.5% phosphoric acid（gradient elution） at the flow rate of 1 ml/min. The column tempera- ture was 35 ~C, and detective wavelengh was set at 327 nm. The similarity of samples was evaluated by using TCM chromatograph- ic Fingerprint Similarity Evaluatio System （2004 A edition）. RESULTS： HPLC fingerprints of L. macranthoides from 28 producing areas of Chongqing Xiushan area was established with good similarity （〉0.96） and separation; 8 peaks in the chromatogram were common, which was in line with the requirements of TCM chromatographic fingerprints. CONCLUSIONS： The method is accurate and reliable, and can provide scientific basis for the quality control of L. macranthoides.