水飞蓟宾对人膀胱癌细胞系T24和5637的增殖抑制及凋亡诱导作用

Inhibitory effects of silibinin on proliferation and apoptosis induction of human bladder cancer T24 and 5637 cell lines

目的:探讨水飞蓟宾(SB)对人膀胱癌细胞系T24和5637增殖及凋亡的影响,初步阐明其可能的机制。方法:培养人膀胱癌细胞系5637和T24,取处于对数生长期的5637和T24细胞分为对照组(0μmol·L-1SB)及25、50、100、200和400μmol·L-1 SB组,MTT法检测SB对人膀胱癌细胞系T24和5637的增殖抑制作用;采用倒置显微镜观察不同浓度SB作用不同时间对人膀胱癌细胞系T24和5637的侵袭抑制能力;DAPI染色法观察给药后细胞形态;流式细胞术检测并评估SB诱导肿瘤细胞凋亡的能力。结果:MTT法检测,随着SB浓度的增加和作用时间延长,不同浓度SB组T24和5637细胞存活率显著低于对照组;不同浓度SB处理后,各组细胞的迁移明显受到抑制;DAPI染色及流式细胞术检测,不同浓度SB组T24和5637细胞凋亡率显著高于对照组(P<0.05)。结论:SB通过诱导人膀胱癌细胞系T24和5637凋亡从而抑制细胞侵袭及增殖。

Objective To determine the effects of silibinin（SB） on the proliferation and apoptosis of human bladder cancer T24 and 5637 cell lines, and to clarify the possible mechanism. Methods Human bladder cancer 5637 and T24 cell lines were cultured in cell media and the 5637 and T24 cells at logarithmic growth phase were divided into control group（0 μmol· L^-1 SB） and 25, 50, 100, 200, and 400 μmol · L^-1 SB groups. The inhibitory effects of SB on the proliferation of T24 and 5637 cells were detected by MTT assay; inverted phase contrast microscope was used to observe the invasion inhibition ability of T24 and 5637 cell lines after treated with different contentations of SB; the morphological changes of T24 and 5637 cells after treatment were observed by DAPI staining; the apoptosis was detected and evaluated by flow cytometry. Results The MTT results showed that the viability rates of T24 and 5637 cell lines were decreased in different concentrations of 8]3 groups in a time-and concentration-dependent manner compared with control group. After treated with different concentrations of SB, the migration of T24 and 5637 cell lines was significantly inhibited. The DAPI staining and flow cytometry results revealed that the apoptotic rates of T24 and 5637 cells in different concentrations of SB groups were higher than those in control group （P〈0.05）. Oonclusion SB can inhibit the proliferation and invasion of human bladder cancer T24 and 5637 cell lines through inducing the apoptosis.