结直肠癌患者手术前后肠道菌群变化分析

Analysis on the Enteric Bacterium Flora Change of Patients before and after Surgery for Colorectal Carcinoma

为通过观察结直肠癌患者手术前后肠道中双歧杆菌（Bifidobacteriumspp．）、乳酸杆菌（Lactobacillus）、粪肠球菌（Enterococcus如ecalis）和拟杆菌（Bacteroidaceae）的变化情况，分析肠道菌群的改变在结直肠癌发病及治疗中的作用及意义，收集正常健康人粪便标本60份、结直肠癌患者术前及术后粪便标本各60份，应用平板活菌计数法和荧光定量聚合酶链反应（PCR）法检测粪便标本中各种菌群的数量，同时采集患者的外周血标本，应用显色法检测D-乳糖和内毒素的含量。结果显示，结直肠癌患者术前粪便中乳酸杆菌、双歧杆菌数量均比正常健康人少，P〈O．05或P〈O．01；粪肠球菌、拟杆菌数量均比正常健康人多，P〈O．05或P〈O．01。结肠癌患者术后粪便中乳酸杆菌、双歧杆菌数量进一步减少，P〈0．05或P〈0．01；粪肠球菌、拟杆菌数量进一步增多，P〈O．05或P〈O．01。结直肠癌患者血清I）I乳糖、内毒素含量比正常健康人高，P〈O．Ol，手术后其含量进一步升高，P〈O．Ol。结果表明，肠道菌群的变化与结直肠癌的发生、发展及预后有一定关系。

Through observation on the change status of the enteric bacterium flora such as Bifidobacteriurn spp. , Lactobacillus , Enterococcus faecalis and Bacteroidaceae this study was to analyzed the action and significance of enteric bacterium flora change in the pathogenesis and treatment of co｜orectal carcinoma. Authors collected 60 feces specimen from health person, 60 preoperative feces specimen and 60 postopera= tive feces specimen from colorectal carcinoma patients, by using plate-mehtod for counting viable bacterium and fluorescent quantitative polymerase chain reaction（PCR） detected the contents of each flora of those specimen;at this time, collected the peripheral blood specimen of patients, by using chromogenie test detec- ted the contents of D-lactose and endotoxin. As results, the contents of Lactobacillus and Bifidobacterium sppl from fecel specimen of patients before surgery were lower than that of health person（ P 〈0.05 or P 〈0.01） ,also further decreased after operation（ P 〈0.05 or P 〈0.01）, but the contents of Enterococcus faecalis and Bateroidaceae higher（ P 〈0. 05 or P 〈0.01） ~also further increased（ P 〈0. 05 or P 〈0. 01） ; the contents of peripheral serum D-lactose and endotoxin in patients were higher than that in health person （ P 〈0.01） ,also further increased after operation（ P 〈0.01）. Results show that the change of enteric bac- terium flora is related tO the pathogenesis,develol0rnent and prognosis of colorectM cancer certainly.