酸性染料比色法测定不同种质槟榔总生物碱的含量

Determination of Total Alkaloids from Different Arecanut( Areca catechu Linn)Germplasm by Acid Dye Colorimetry Method

目的:建立一种简便且准确测定槟榔中总生物碱含量的方法。方法:以氢溴酸槟榔碱为对照品,采用酸性染料比色法测定总生物碱的含量,测定波长为618 nm。结果:槟榔总生物碱在20~120μg呈线性关系,回归方程为Y=4.226 7 X-0.005 2(r=0.999 6),平均回收率为99.6%,RSD=1.54%。稳定性、精密度和重复性都比较高,RSD分别为1.32%,0.109%,2.18%。样品分析结果为红心槟榔含量最高,常见槟榔含量次之,西瓜槟榔较低。结论:该方法操作简便、灵敏、准确,可用于槟榔总生物碱的质量控制。

Objective： To establish a simple and accurate method for the determination of total alkaloids in arecanut. Methods： The determination was carried out by acid dye colorimetry with arecoline hydrobromide as reference, at wavelength of 618 nm on UV-2450 spectrophotometer. Results： The total alkaloid contents of arecanut were linear in the range of 20 to 120 p^g, the regression equation was Y = 4. 226 7X -0. 005 2 （r =0. 999 6） , the average recovery was 99.6% with a RSD of 1.54%. There was a relatively high stability, precision and repeatability, RSD were 1.32%, 0.109% and 2.18% respectively. The results of sample analysis showed the highest total alkaloid content was in red arecanut germplasm, followed by common arecanut germplasm, the lowest in watermelon arecanut germplasm. Conclusion： The method is simple, sensitive and accurate.