复发性自然流产患者外周血中Th17及Treg细胞及其效应因子的变化

The Changes of Th17 and Treg Cells in the Recurrent Spontaneous Abortion Patients

目的:探讨复发性自然流产患者外周血中Th17、Treg细胞以及相关细胞因子的变化。方法:选择复发性自然流产患者25例,正常妊娠妇女25例以及正常非妊娠育龄妇女25例,流式细胞术测定外周血中Th17和Treg细胞数量,ELISA法测定血清IL-10、TGF-β及IL-17的浓度。结果:复发性自然流产患者外周血中Th17细胞百分率显著高于正常妊娠以及正常非孕妇女(P<0.05),Treg细胞百分率显著低于正常非孕妇女(P<0.05),但与正常妊娠妇女相比无显著性差异(P>0.05);复发性自然流产患者外周血IL-10及TGF-β水平显著低于正常妊娠妇女以及正常非孕妇女(P<0.05),而IL-17水平显著高于正常妊娠妇女以及正常非孕妇女(P<0.05)。结论:外周血Th17细胞数和IL-17水平的升高以及抑制性细胞因子IL-10和TGF-β水平的下降可能是复发性自然流产发生的重要原因。

Objective： T he changes of Thl 7 cells, Treg cells and related cytokines in peripheral blood of recurrent spontaneous abortion （RSA） patients was discussed. Methods： 25 recurrent spontaneous abortion patients, 25 normal pregnant women and 25 normal non-pregnant women at childbearing age were selected in this study, The numbers of Thl7 and Treg cell in peripheral blood were measured by flow cytometry. The concentrations of serum IL-10, TGF- β, and IL-17 were determined by ELISA kits. Results： The percentage of Thl7 cell in peripheral blood of RSA patients was significantly higher than that of normal pregnant and normal non-pregnant women（P〈0.05）, the percentage of Treg cell in peripheral blood of RSA patients was significantly lower than that in normal non-pregnant women（P〈0.05）, but there was no significant difference in comparison with normal pregnanct women（P〉0.05）. The levels of serum IL-10 and TGF-β of RSA patients were significantly lower than that of normal pregnant women and normal non-pregnant women（P〈0.05）, while IL-17 level was significantly higher than that in normal pregnant and non-pregnant women（P〈0.05）. Conclusion： The number of Thl7 cell, IL-17 level and serum level of inhibitory cytokines IL-10 and TGF-beta may play an important role in the development of recurrent spontaneous abortion.