驱油生物表面活性剂生产菌的筛选与评价

The Evaluation and Screening of Oil-displacing Biosurfactant-producing Bacteria

目的:筛选适合油田的生物表面活性剂生产菌。方法:通过发酵培养,研究生物表面活性剂生产菌生长代谢规律;采用正交试验法,优选出其最佳培养条件;通过室内驱油实验评价生物表面活性剂驱油效果。结果:2#菌株最佳培养时间为96小时,最优发酵培养条件为:葡萄糖4.0 g、玉米浆1.6 g、Na2HPO40.1 g、KH2PO40.05 g、MgSO40.05 g、CaCl20.005 g、水100 mL、pH 7.2,培养温度35℃,摇床转速120 r/min,生物表面活性剂驱油提高采收率6.16%。结论:筛选出最优生物表面活性剂产生菌2#,菌株具备产表面活性剂的能力且产物量较高,其生物表面活性剂驱油效果良好。

Objective： To filter out biosurfactant producing strain for oilfield. Methods： To study on the growth and metabolism law ofbiosurfactant-producing bacteria was studied by the fermentation, and the best culture conditions were elected by using orthogonal experimental method. The biological surfactant oil displacement effect was evaluated through laboratory displacement experiment. Results： The best culture time for strain 2# was 96 hours. The optimal condition for fermenting were as follows： the fermentation medium was composed of 4.0 g of glucose, 1.6 g of corn steep liquor, 0.1 g of Na2HPO4, 0.05 g of KH2PO4, 0.05 g of MgSO4, 0.005 g of CaCl2, 100 mL of water and pH 7.0; The culturing temperature was 35 ℃; The rotation speed of shaking table was 120 r/min. Biosurfactant flooding EOR efficiency was 6.16 %. Conclusions： The best oil-displacing biosurfactant-producing bacteria is strain 2#. It could produce surfactant and the product quantity is high. The effect ofbiosurfactant flooding is good.