环氧合酶-2在原发及复发星形细胞瘤中的表达及意义

The expression and significance of cyclooxygenase-2 in primary and recurrent astrocytoma

目的 通过免疫组织化学方法,观察环氧合酶（COX）-2在不同恶性程度星形细胞瘤中的表达,从而对COX-2在星形细胞瘤中可能的侵袭作用做出初步判定.方法 选择行手术治疗的星形细胞瘤患者55例,共行63次手术,其中8例复发行二次手术.选择同期9例重度颅脑外伤必须行减压手术的患者作为对照.将术中取出的星形细胞瘤组织标本及切除的减压组织标本立即使用10％甲醛缓冲溶液固定,采用免疫组织化学方法检测COX-2的表达情况.结果 星形细胞瘤组织COX-2阳性表达率[69.84％ （44/63）]高于正常脑组织（1/9）,差异有统计学意义（r=11.589,P＜0.01）.复发星形细胞瘤组织COX-2阳性表达率（8/8）高于原发星形细胞瘤组织[65.45％（36/55）],差异有统计学意义（Х^2=3.957,P＜ 0.05）.随星形细胞瘤恶性程度增高,COX-2阳性表达率也随之增高,差异有统计学意义（P＜ 0.05）.结论 COX-2可能与星形细胞瘤的侵袭有一定关系,从而导致了肿瘤病理分级的增高以及复发.

Objective To observe the expression of cyclooxygenase（COX）-2 in different malignancy astrocytoma by immunohistochemistry,so as to judge the invasive action of COX-2 in astrocytoma.Methods A total of 55 cases with astrocytoma underwent 63 times operation including 8 recurrent cases were selected,and 9 cases with severe brain injury who needed decompression surgery were selected as control group.The astrocytoma tissues and decompression surgery to remove tissues removed immediately fixed in 10% formalin buffer solution.The expression of COX-2 was determined by immunohistochemistry.Results The positive expression rate of COX-2 in astrocytoma tissues was higher than that in decompression surgery to remove tissues[69.84% （44/63） vs.1/9],and there was significant difference （Х^2 =11.589,P 〈 0.01）.The positive expression rate of COX-2 in recurrent astrocytoma tissues was higher than that in primmy astrocytoma tissues [8/8 vs.65.45% （36/55）],and there was significant difference （Х^2 =3.957,P 〈 0.05）.With increased astrocytoma malignancy,the positive expression rate of COX-2 also increased,and there was significant difference （P 〈 0.05）.Conclusion COX-2 may have some relationship with the invasion of astrocytoma,leading to increase tumor grade,and relapse.