我国部分地区鸡感染禽坦布苏病毒血清流行病学调查

Serological Detection and Analysis of Chicken-origin Avian Tembusu Virus Infection in Some Parts of China

为了解鸡群感染禽坦布苏病毒的情况,应用已建立的间接酶联免疫吸附试验(间接ELISA)对2010年12月至2013年9月近3年内采自福建、江西、浙江、广东4省发生产蛋异常鸡场的开产海兰蛋鸡、后备海兰蛋鸡2 300份血清样品进行禽坦布苏病毒抗体的检测。结果显示,开产蛋鸡血清样品的禽坦布苏病毒抗体阳性率为43.4%,而后备蛋鸡为19.9%;在开产蛋鸡中,以福建省的阳性率最高,为60.7%,而在后备蛋鸡中则以广东省最高,为37.2%。4省采集的蛋鸡血清样品中均检出禽坦布苏病毒抗体,且开产蛋鸡的阳性率高于后备蛋鸡,揭示福建、江西、浙江、广东4省海兰蛋鸡在不同程度上感染禽坦布苏病毒,研究结果可为以上地区乃至我国鸡群做好禽坦布苏病毒感染的预防提供依据。

To investigate avian Tembusu virus infection in chickens ,2 300 sera samples were collected from highland laying hens and reserve hens in Fujian ,Jiangxi ,Zhejiang and Guangdong provinces during Dec .2010 to Sep .2013.The antibodies were detected by indirect enzyme-linked immuosorbent assay （ELISA） .The results showed that the samples of laying hens had higher seropositive rate （43.4% ） than that of reserve hens （19.9% ） .For laying hens , specimens from Fujian province had the highest seropositivity （60.7% ） ,and for reserve hens the specimens from Guangdong province had the highest seropositivity （37.2% ） .The antibodies for avian Tembusu virus were detected from serum samples in all four provinces with different antibody positive rate ,and the samples of laying hens had higher seropositive rate than reserve hens .The serological investigation indicated that different degrees of avian Tembusu virus infection existed in all these regions ,providing the scientific basis for preventing avian tembusu virus infection in chickens in these regions and even in China .