摘要
利用弱酸性离子树脂HZ-830对绣球菌多糖进行脱色,通过Box-Benhnken中心组合试验和响应面分析法,在前期单因素试验基础上,以脱色温度、脱色pH和脱色时间为自变量,脱色率为响应值,将HZ-830树脂对绣球菌多糖的脱色工艺进行优化。优化后确定的最佳脱色工艺条件为:脱色温度(A)=41℃,pH(B)=8,脱色时间(C)=3.5h,平均脱色率为87.73%。
In this paper , response surface methodology was applied to optimize resin decoloration technology of Sparassis crispa polysaccharide by type HZ-830. On the basis of single factor test ,the mathematical regression model was established about the dependent variable (decolorization rate ) and independent variables (decoloration temperature ,pH ,decoloration time) . The results showed that the optimum decolorizing conditions of Sparassis crispa polysaccharide by resin HZ-830 were as follows :decolorizing temperature 41℃ ,pH8 ,decolorizing time 3.5h .Under this condition ,the decolorizing rate could reach 87.73% .
出处
《福建农业学报》
CAS
2014年第1期67-71,共5页
Fujian Journal of Agricultural Sciences
基金
国家支撑计划课题(2012BAD14B15)
福建省自然基金项目(2010J01100)
关键词
绣球菌
多糖
脱色
树脂
响应面法
Sparassis crispa
polysaccharide
decoloration
resin
response surface methodology