二乙三胺锌(Ⅱ)配合物对水解酶的模拟研究

Studies of Artificial Hydrolytic Metalloenzymes

研究了二乙三胺 (Dien)锌 (Ⅱ )配合物作水解酶模拟物催化对硝基苯酚醋酸酯 (NA)水解的动力学。结果表明 :催化水解速率对底物 (NA)及配合物 (ZnDien)浓度均呈一级反应 ,水解反应遵循速率方程dC/dt=(kobs[Zn Dien]+KOH[OH-]+… ) [NA],催化反应受酸碱平衡控制 ,催化机理与天然酶一致 ,表明了该项研究设计的配体和锌酶中配位环境与天然酶相似的特点。

The hydrolytic kinetics of p nitrophenol acetate (NA) catalyzed by zinc(Ⅱ) complex of Polyamine (Dien) has been studied.It shows that the catalytic hydrolytic rate is of first order to both zince(Ⅱ) complex and NA concentrations.The hydrolysis proves to be catalytic:d C /d t =( k [ZnDien]+ K OH [OH -]+…)[NA].This is one of the most efficient hydrolytic metalloenzyme model compounds reported up to now.