摘要
Objective： To investigate whether pinching spine （PS, i.e.捏脊, a traditional Chinese manipulativetherapy） is beneficial to ameliorating the depressive state （including behavioral deficit, retardative weight gainand decreased sucrose consumption） in a rat model of depression induced by chronic unpredictable stress （CUS）and to explore the candidate mechanism of action. Methods： PS was performed on rats＇ spine once daily for 1week after exposure to CUS. The open-field test, body weight measuring, and sucrose intake test were appliedon different dates： before stress （dO）, at the end of stress （d21） and after PS treatment （d28）, respectively. Thenthe rats＇ hippocampuses were performed genome-wide microarray analysis, and the expression levels of severalgenes were evaluated by real-time polymerase chain reaction （PCR）. Results： Exposure to CUS resulted indecreases of behavioral activity and sucrose consumption, which were reversed significantly after PS treatment.The expression of several genes relevant to energy metabolism, anti-oxidation, and olfactory receptor, etc., weredown-regulated, while the expression of those relevant to hemostasis, immunity-inflammation, and restrictionof activities and ingestion, etc., were up-regulated in hippocampuses of rats exposed to CUS. PS treatmentsignificantly inverted these changes. Furthermore, increase or decrease in gone expression evaluated by real-time PCR was concordant with up-regulated or down-regulated expression evaluated by microarray analysis.Conclusion： PS showed a potential antidepressant-like effect, of which the action mechanism might be due togone expression regulation in hippocampus.
Objective： To investigate whether pinching spine （PS, i.e.捏脊, a traditional Chinese manipulativetherapy） is beneficial to ameliorating the depressive state （including behavioral deficit, retardative weight gainand decreased sucrose consumption） in a rat model of depression induced by chronic unpredictable stress （CUS）and to explore the candidate mechanism of action. Methods： PS was performed on rats＇ spine once daily for 1week after exposure to CUS. The open-field test, body weight measuring, and sucrose intake test were appliedon different dates： before stress （dO）, at the end of stress （d21） and after PS treatment （d28）, respectively. Thenthe rats＇ hippocampuses were performed genome-wide microarray analysis, and the expression levels of severalgenes were evaluated by real-time polymerase chain reaction （PCR）. Results： Exposure to CUS resulted indecreases of behavioral activity and sucrose consumption, which were reversed significantly after PS treatment.The expression of several genes relevant to energy metabolism, anti-oxidation, and olfactory receptor, etc., weredown-regulated, while the expression of those relevant to hemostasis, immunity-inflammation, and restrictionof activities and ingestion, etc., were up-regulated in hippocampuses of rats exposed to CUS. PS treatmentsignificantly inverted these changes. Furthermore, increase or decrease in gone expression evaluated by real-time PCR was concordant with up-regulated or down-regulated expression evaluated by microarray analysis.Conclusion： PS showed a potential antidepressant-like effect, of which the action mechanism might be due togone expression regulation in hippocampus.
