摘要
以2.硫代巴比妥酸(TBA)修饰金纳米粒子为探针,TBA与三聚氰胺通过氢键作用诱导金纳米探针团聚,进而使金纳米胶体颜色由酒红色变为蓝色。实验优化得最佳反应条件为在乙酸缓冲溶液(pH=7.0)介质中,室温反应15min。对不同浓度三聚氰胺进行检测时发现,在0.062~0.18 μmol/L和0.18~6.0 μmol/L之间,A。/A,如吸收比率与三聚氰胺浓度呈现良好的线性关系,检出限为0.043 μmol/L。该方法用于检测牛奶样品中的三聚氰胺的加标回收率为102.8%~105.3%。
A simple and rapid colorimetric method was developed for the detection of melamine using 2-thiobarbituric acid-modified gold nanoparticles (TBA-AuNPs) as probes. Melamine can induce the aggregation of TBA-AuNPs via the hydrogen-bonding interactions in acetic acid buffer solutions ( pH = 7 ) , resulting in the color change from wine-red to blue within 15 min at room temperature. Under optimized conditions, the absorption ratio of the A660/A520 shows good linear relationship with the melamine concentration in the range of 0.062 ~ 0. 18 μmol/L and 0.18 ~ 6.0 μmol/L, respectively, with the detection limit of 0. 043 μmol/L. The recoveries of the method are within the range of 102.8% ~ 105.3%. It provides a platform for rapid, selective and sensitive colorimetric detection of melamine in milk samples.
出处
《应用化学》
CAS
CSCD
北大核心
2014年第4期496-501,共6页
Chinese Journal of Applied Chemistry
基金
国家自然科学基金资助项目(21175118
21275130)~~
关键词
三聚氰胺
硫代巴比妥酸
金纳米探针
比色法
melamine
thiobarbituric acid
gold nanoparticles probes
colorimetric method