莞香叶芒果总苷的制备及其抗炎镇痛药效学研究

Effects of Total Mangiferin from Guanxiang Leaves on Anti-inflammation and Abirritation

目的从莞香叶中分离得到芒果总苷,并研究该部位抗炎镇痛药效学作用,为莞香资源的药效开发利用提供实验依据。方法通过一系列分离纯化手段,得到莞香叶芒果总苷部位。通过醋酸致小鼠腹腔毛细血管通透性模型与二甲苯致小鼠耳肿胀模型,评价莞香叶芒果总苷的抗炎药效学作用,通过小鼠热板模型与醋酸致小鼠疼痛模型,评价莞香叶芒果总苷的镇痛药效学作用。结果与空白对照组比较,莞香叶芒果总苷高、中剂量给药能够显著抑制小鼠腹腔毛细血管通透性,并能够显著提高小鼠热板法痛阈百分率(P<0.05);莞香叶芒果总苷各剂量组均可显著降低二甲苯致炎小鼠的耳肿胀度以及显著减少小鼠20min内扭体次数(P<0.05)。结论莞香叶芒果总苷对于上述4种动物模型均具有显著的药理作用,证明莞香叶芒果总苷具有显著的抗炎镇痛药效。

Objective To investigate the anti -inflammation and abirritation effect of Total mangiferin extracted from GuanXiang leaves. To provide experimental support for developing drugs from Aquilaria sinensis（Loun） Gilg source. Methods Total mangiferin from GuanXiang leaves was extracted by a serial separation, purification method. The ear swelling degree induced by dimethylbenzene and the capillary permeability in abdominal cavity induced by acetic acid were used to evaluate anti - inflammation effect of total mangiferin from GuanXiang leaves. To evaluate the effect of Abirritation, two animal modes - hot plate tests and acetic acid stretching experiment in mice were used. Results Compared with control group, high and mid dose of total mangiferin from Guanxiang leaves can effectively suppress the capillary permeability in abdominal in mouse and increase the percent of pain in the limitation in the hot - plate tests （ P 〈 0.05 ）. All doses tested can effectively reduce auricles inflammation resulted from xylene and reduce times of writhing in 20min（P 〈 0.05 ） observa- tion time. Conclusion Total mangiferin from Guanxiang leaves is effective in all the four animal models tested which suggest that total mangiferin from Guanxiang leaf has anti - inflammation and abirritation effect.