云斑天牛对10种植物挥发物的EAG和行为反应

EAG and behavioral responses of Batocera lineolata Chevrolat(Coleoptera: Cerambycidae) to ten plant volatiles

【目的】为了探明对云斑天牛Batocera lineolata Chevrolat成虫具有行为导向的寄主植物挥发物对成虫行为反应的影响。【方法】采用触角电位(EAG)和"Y"型嗅觉仪技术,测定云斑天牛成虫(未交配雌虫、未交配雄虫、已交配雌虫)对10种寄主植物挥发物的电生理和行为反应。【结果】所选化合物在特定浓度下,均能引起云斑天牛成虫较为明显的EAG反应,浓度为100μg/μL时,云斑天牛成虫对所测试的植物挥发物的EAG反应最强,其中未交配雌、雄及已交配雌虫对(Z)-3-己烯-1-醇的EAG反应相对值均为最大,分别达2.35、2.31和2.22。行为反应生测中,(Z)-3-己烯-1-醇对未交配雌、雄虫可产生明显的引诱作用,芳樟醇则对两者具有明显的趋避作用,而已交配雌虫对所有化合物均无明显趋性。【结论】试验结果可为开发对其有效的林间引诱剂提供一定的理论依据。

[Objectives] To verify the effects of the host plant volatiles on the behavior of adult Batocera lineolata Chevrolat. [Methods] The electroantennogram and behavioral responses of the B. lineolata adults （unmated females, unmated males and mated females） to 10 kinds of plant volatiles were tested with EAG and a Y-tube olfactometer. [Resultsl The ten test chemicals induced clear EAG responses. B. lineolata adults had the strongest EAG responses to the tested plant volatiles when the concentration was 100 μg/μL. The EAG responses values of unmated female and male and mated female B. lineolata adults to （Z）-3-Hexen-1-ol were the highest, being 2.35, 2.31 and 2.22, respectively. As for behavioral responses, unmated female and male B. lineolata adults were strongly attracted to （Z）-3-Hexen-l-ol, whereas Linalool had an obvious repellent effect on unmated female and male B. lineolata adults. None of the compounds tested were attractive to mated female B. lineolata adults. [Conclusion] The results provide some theoretical basis for the development of effective attractants for B. lineolata.