昆虫触角电位(EAG)及其与气谱联用(GC-EAD)技术

EAG and GC-EAD techniques

昆虫触角电位(EAG)和气相色谱-触角电位(GC-EAD)是昆虫化学生态学中最重要的电生理技术,在研究昆虫对信息化学物质的感受机理、筛选活性信息素成分或植物次生物质等方面发挥关键作用。本文介绍这两种技术的基本原理、操作步骤和应用实例等,并对使用中应该注意的事项进行了详尽讨论。

Electroantennography （EAG） and gas chromatography-electroantennographic detection （GC-EAD）, two of the most important techniques in insect chemical ecology, play key roles in the study of insect chemoreception mechanisms, the screening of bioactive components of insect pheromones or plant allelochemicals, and so on. This paper describes technical principles, detailed operational procedures and examples of EAG and GC-EAD techniques, and discusses some key points in the application of these two techniques.