一种基于DPA识别基团的可视化检测锌离子的荧光增强型探针

A Fluorescence Enhancement Probe for Visual Detection of Zn^(2+) with Receptor Based on DPA

以N,N-二(2-吡啶甲基)胺(DPA)为识别基团,设计合成了一种用于检测Zn2+的荧光增强型探针WN,在CH3CH2OH/TrisHCl(1∶9,V/V,pH=7.4)缓冲溶液中研究了它对Zn2+的识别特性。实验结果表明,WN对Zn2+有较高的选择性和灵敏度,它们之间的结合比为1∶1,对Zn2+的检出限为1.14×10-8 mol·L-1。WN能够快速地可视化检测Zn2+,在HeLa活细胞中对Zn2+的荧光显微成像表明WN可应用于生物体的检测。

A fluorescence enhancement probe WN for detection of Zn2＋ with receptor based on di-2-picolylamine （DPA） was synthesized and structurally characterized. Its recognition properties with Zn2＋ had been investigated in CH3CH2OH/Tris-HCl buffer solution （1：9, V/V, pH=7.4）. The results showed that WN exhibited good sensitivity and selectivity to Zn2＋, and the stoichiometry of WN-Zn2＋ complex was estimated to be 1：1, the detection limit of Zn2＋ was estimated to be 1.14×10^-8 mol.L-1. WN was used for fast visual detection of Zn2＋ and its intraeelluar Zn2＋ imaging in living HeLa cells, indicated that it could be applied in biological detection. CCDC： 960510