CMV启动子克隆及其在植物体内的表达

Cloning and expression study of CMV promoter in plant

根据细胞肥大病毒CMV启动子基因序列设计引物,PCR扩增目的基因后,利用基因重组技术成功构建具有Kan抗性和GUS intron报告基因的植物表达载体LpPCG,并将重组质粒转化到烟草叶片中.通过CMV启动子指导的GUS intron基因在烟草叶片内的瞬时性表达,比较了其植物表达特性.结果表明:CMV启动子可启动GUS在植物体内的表达,其表达活性相当于2×35S启动子的(80.4±26.6)%.

Based on the CMV promoter gene sequence, a pair of primers was designed,and the target gene was amplified by PCR. The recombinant expression plasmid LpPCG which contained Kanamycin re- sistance and a GUS intron reporter gene was constructed and then transformed into tobacco leaves. Compa- ring its plant expressional activity through GUS intron transient expression which guided by CMV promot- er in the tobacco leaves. The results showed that CMV promoter could drive GUS expression in plants. Its expression activity was equivalent to（80.4±26.6）% of the 2 × 35S promoter.