肠艾舒对小鼠CT-26大肠癌组织Ki67和PCNA表达的影响

Effect of Chang'aishu on Expression of Ki67 and PCNA in Mice Model with CT-26 Colorectal Cancer

目的观察肠艾舒对荷瘤小鼠CT-26大肠癌的抑瘤作用及其对肿瘤组织Ki67、PCNA表达的影响。方法将40只BALB/c小鼠随机分为4组,右前腋下接种CT-26细胞造模,空白组(NS 0.6 mL)、化疗组(卡培他滨205.5 mg/kg)、中药高剂量组(肠艾舒51.38 g/kg)、中药低剂量组(肠艾舒17.13 g/kg)隔日灌胃,15 d后计算抑瘤率,免疫组化法检测肿瘤组织Ki67、PCNA的表达。结果与空白组比较,化疗组、中药高剂量组、中药低剂量组抑瘤率分别为43.35%、29.48%、13.30%;与空白组比较,各治疗组Ki67、PCNA标记指数差异均有统计学意义(P<0.05,P<0.01)。结论肠艾舒对小鼠CT-26大肠癌的体内生长有一定抑制作用,可能与其下调肿瘤组织Ki67、PCNA表达有关。

Objective To observe the anti-tumor action of Chang＇aishu and its influence on the expression of the Ki67 and PCNA. Methods Forty Balb/c mice with CT-26 colorectal cancer were randomly divided into four groups, including the control group （normal saline, 0.6 mL）, the chemotherapy group （Xeloda 205.5 mg/kg）, the high dose group （Chang＇aishu 51.38 g/kg） and the low dose group （Chang＇aishu 17.13 g/kg）. Each group of mice was treated with intragastric administration every two days. After 15 days, the anti-tumor rate was calculated and the expression of Ki67 and PCNA were analyzed by immunohistochemistry. Results Compared with the control group, inhibitive rate of the chemotherapy group, the high dose group and the low dose group was 43.35%, 29.48% and 13.30%, respectively. Compared with the control group, the treatment group showed statistical significant difference in Ki67 and PCNA （P〈0.05, P〈0.01）. Conclusion Chang aishu had inhibition effect on the growth of colorectal cancer, which may be related to down-regulating the expression of Ki67 and PCNA.