HPLC测定元胡止痛软胶囊中没食子酸含量

Determination of Gallic Acid in Yuanhu Zhitong Soft Capsule by HPLC

目的：建立元胡止痛软胶囊中没食子酸含量的高效液相色谱测定方法。方法色谱柱：Unitary C18（250 mm×4.6 mm,5μm）；流动相：甲醇-0.5%磷酸（5∶95）；流速：1.0 mL/min；柱温：30℃,检测波长：254 nm。结果没食子酸在0.0248～0.496μg范围内与峰面积呈良好的线性关系,r＝0.9999,日内精密度、重复性、稳定性的RSD分别为0.27%、0.21%、1.40%,平均加样回收率为99.81%,RSD＝0.72%。结论本方法精确可靠,可作为元胡止痛软胶囊中没食子酸含量测定的方法。

Objective To establish the determination method of gallic acid in Yuanhu Zhitong Soft Capsule by HPLC. Methods Chromatography was performed on a Unitary C18 column （250 mm× 4.6 mm, 5 μm） at 254 nm. The mobile phase consisted of a mixture of methanol and 0.5%phosphate acid （5∶95） at flow rate of 1.0 mL/min. Results The standard curve of linear relationship was 0.024 8-0.496 μg and r=0.999 9. The RSD of precision, repeatability and stability was 0.27%, 0.21%, 1.40%, respectively. The average recovery was 99.81%, RSD was 0.72%. Conclusion The established method was accurate and reliable for the determination of gallic acid in Yuanhu Zhitong Soft Capsule.