宿主p53基因第4外显子72位密码子多态性(Pro/Arg)与HPV16感染的相关性研究

Association study of polymorphism(Pro/Arg) within 72 codon of exon 4 of Host p53 gene with HPV16 infection

目的：研究宿主p53基因密码子72多态性（Pro/Arg）在HPV16感染者和正常健康人群中的分布情况。方法采用 TaqMan SNP Genotyping Assays 方法进行 p53基因密码子72（Pro/Arg）多态性分析。结果 p53基因密码子72（Pro/Arg）突变的基因型和等位基因频率在HPV16感染组和对照组中差异无统计学意义（P＝0.30和P＝0.88）。不同遗传模型分析显示，p53基因密码子72（Pro/Arg）多态性在HPV16感染组和对照组中差异无统计学意义（P 0.12～0.88）。结论本研究未发现宿主p53基因密码子72（Pro/Arg）多态性与HPV16感染相关，结合其他报道数据，推测该突变可能在宫颈正常细胞向癌症细胞转化中起到重要作用。

Objective To investigate association study of host p53 gene codon 72 polymorphism （Pro/Arg） with HPV 16 infection. Methods p53 gene codon 72 polymorphism（Pro/Arg） was genotyped by TaqMan SNP Genotyping Assays. Results The frequency of genotype and allele of codon 72（Pro/Arg） was not statistically significant（P=0.30 and P=0.88） between HPV16 infectious and control group. Different genetic model analysis showed that the polymorphism had no significant difference between the two groups（P=0.12 to 0.88）. Conclusion This study observe that host p53 gene codon 72 polymorphism（Pro/Arg） is not associated with HPV16 infection, but might play an important role in transformation of normal cells to cervical cancer cells.