包裹RuBpy二氧化硅荧光纳米探针的制备及其用于肝癌细胞的识别

Preparation of RuBpy-doped Silica Fluorescent Nanoprobes and Their Applications to Recognition of Liver Cancer Cells

制备了两种不同官能团修饰的偶联亲和素的包裹钌联吡啶(RuBpy)二氧化硅荧光纳米探针A和探针B,并分别用于肝癌细胞的识别。通过反相微乳液法制备得到表面修饰不同官能团的纳米颗粒,然后通过亲和素与羧基化包裹RuBpy二氧化硅纳米颗粒相互连接而制备得到探针A;通过亲和素与PEG修饰的荧光二氧化硅纳米颗粒相互作用而制备得到探针B。与探针A不同的是,探针B通过一个长链PEG分子将亲和素与荧光二氧化硅纳米颗粒偶联在一起。利用免疫荧光成像法将这两种探针分别用于人肝癌细胞的识别,结果表明,含有长链PEG分子的探针B更能够有效地识别肝癌细胞表面肿瘤标志物癌胚抗原(CEA)。

Two kinds of different functional groups modified RuBpy-doped silica fluorescent nanoprobes Probe A and B that conjugated with avidin were prepared for the recognition of liver cancer ceils. Firstly RuBpy- doped silica nanopartieles were synthesized by reverse microemulsion and modified with different functional groups, then Probe A was prepared by the conjugation of avidin with carboxyl modified nanoparticles through covalent binding using 1-ethyl-3-（ 3-dimethylamino propyl）carbodiimide hydrochloride （EDC）/sulfo-NHS, whereas Probe B was prepared by the conjugation of avidin with the polyethylene glycol （PEG） linkers on the surface nanoparticles using cyanogen bromide method. Therefore, compared with Probe A, Probe B was obtained by coupling avidin to the nanoparticles through long-chain PEG molecules. The two probes were incubated with liver cancer cells respectively, and microscopic fluorescence imaging shows that Probe B which contained PEG molecules could be more effectively applied for the recognition of tumor marker carcinoembryonie antigen （CEA） in liver cancer cells.