透射式扫描近场光学显微镜探针光场分布及其受激荧光分子光场分布研究

STUDY OF THE DISTRIBUTION OF LIGHT INTENSITY OF THE FIBER PROBE OF TRANSMISSION SCANNING NEAR FIELD OPTICAL MICROSCOPY AND THE DISTRIBUTION OF EXCITED FLUORESCENT MOLECULES

通过利用经Grober发展的Bethe模型 ,计算了透射式近场光学显微镜探针针尖附近及进入样品后的光场分布 ,研究了它的横向分辨率、透射深度、透射系数等问题 .模拟在光学近场激发下生物荧光分子成像的过程 ,研究了荧光分子的极化方向和入射光偏振方向对信号收集的影响 ,发现荧光图像是偏振极性和荧光分子极性共同作用的结果 .

The light intensity distribution near the fiber probe of the Transmission Scanning Near Field Optical Microscopy(SNOM) was calculated by the model of R.D.Grober.The resolution,transmission depth and transmittance of the light intensity were discussed.The imaging process of excited biological fluorescent molecules was simulated,and it was found that the dipole direction of fluorescent molecules and the polarization of incident light influenced the detected signals.Both the polarization direction of incident light and the dipole direction of fluorescent molecules would affect the fluorescent molecules imaging.