造纸白水中的细菌多样性及系统发育树分析

Bacterial diversity and phylogenetic analysis of whitewater in papermaking process

在制浆造纸过程中,白水的循环回用加重了纸机微生物污染的问题,促进了纸机腐浆的形成,影响了纸机的正常运转。此次研究用滤膜抽滤富集细菌菌体,对白水样品直接提取DNA,通过16S rDNA序列分析,构建16S rDNA文库,对芬欧汇川(UPM)常熟纸厂PM1纸机白水中的细菌多样性进行了研究。获得的61个16S rDNA序列可分为28个可操作分类单元(OTUs),分属于6个不同门类,优势菌种顺序为变形菌门(Proteobacteria)(77.05%)、厚壁菌门(Firmicutes)(11.48%)、放线菌门(Actinobacteria)(4.92%)、异常球菌-栖热菌门(Deinococcus-Thermus)(3.28%)、拟杆菌门(Bacteroidetes)(1.64%)、蓝藻门(Cyanobacteria)(1.64%)。造纸白水具有较丰富的细菌多样性,以变形菌为优势菌群,占库容总量的77.05%,其中尤以β-变形菌群为主,占库容总量的45.90%,而首次在中国纸机鉴定出的水生施莱格尔氏菌(Schlegelella aquatica)为克隆文库的优势菌株,另外还含有2个菌种属于异常球菌-栖热菌门,该菌门为红色腐浆形成菌种。

Paper machines offer a suitable environment for microbial growth, which results in slime problems during the process of papermaking. Investigation of the bacterial diversity in whitewater which recycled in paper mill UPM was car-ried out in this paper. Total DNA were directly extracted from the whitewater sample. 16S rDNA were amplified directly from purified DNA by PCR with universally bacteria-specific rDNA primers and cloned. Sixty-one bacteria were identified by partial sequencing of their 16S rDNAs. Twenty-eight operational taxonomic units （ OTUs） were clustered in the follow-ing phyla including Proteobacteria （ 77. 05%） , Firmicutes （ 11. 48%） , Actinobacteria （ 4. 92%） , Deinococcus-Thermus （3.28%）, Bacteroidetes（1.64%）,Cyanobacteria（1.64%）. Bacterial diversity was high in the whitewater sample. The results showed a high proportion of Schlegelella aquatica in the white water samples. It was the first time that Schlegelella aquatica strain had been identified from a paper machine in China. We also identified the red-slime formation bacteria Deinococcus-Thermus in whitewater sample.