大叶常绿杜鹃叶片离体培养及不定芽再生

In vitro leave culture of broad leaf evergreen rhododendron and its regeneration of adventitious buds

以大叶常绿杜鹃无菌苗的嫩叶为试验起始材料，研究了其离体培养和不定芽再生的过程。结果表明：培养基为MS＋ZT（1．0 mg/L）＋ NAA（0．1 mg/L）时，大叶常绿杜鹃叶片可直接诱导再生不定芽，诱导率达70％；也可以通过愈伤组织途径间接诱导出不定芽，愈伤组织诱导培养基为MS＋2，4-D（2．0 mg/L）＋KT（0．2 mg/L），增殖最佳培养基为MS＋ZT（0．5 mg/L）＋IBA（0．1 mg/L），愈伤组织分化的最佳培养基为MS＋ZT（0．04 mg/L）＋NAA（0．01 mg/L）；分化率最高为70％，分化苗数为6～10株；生根培养基为MS＋NAA（0．2 mg/L）＋IBA（0．4 mg/L）时，生根率可达90％。腐殖土、黄沙、珍珠岩配比为4∶1∶1的混合基质较适合常绿杜鹃试管苗的炼苗移栽，成活率为90％。

Using the young leaves of aseptic broad leaf evergreen rhododendron seedling as the starting materials for this experiment, the culture conditions in vitro and regeneration of adventitious budswere studied. The results showed that：the best medium composition inducing adventitious buds directly from leaf explants were MS＋ZT 1.0 mg/L＋NAA 0.1 mg/L, which made the induction rate reach to 70%. The adventitious buds could also be induced from the callus indirectly, the best medium composition for which was MS＋2,4-D 2.0 mg/L＋KT 0.2 mg/L, the best multiplication medium for cal-lus was MS＋ZT 0.5 mg/L＋ IBA 0.1mg/L,the best medium for callus differentiation was MS＋ZT 0.04 mg/L＋NAA 0.01 mg/L, which made differentiation rate reach to 70% and the differentiation number of seedlings 6-10. The rate of rooting could reach to 90% by the medium of MS＋NAA 0.2 mg/L ＋IBA 0.4 mg/L. The mixed compost contained four parts of humus soil, and one part of yellow sand, one part of perlite, which was quite suitable for evergreen rhododendron seed-lings transplanting in vitro, and the survival rate was 90%.