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猪伪狂犬病病毒 gE-/gI-/TK-多基因缺失活疫苗对猪的安全性与免疫效力研究 被引量：19

Study on Safety and Immune Efficiency of Pseudorabies Virus Alive Vaccine with gE^-/gI^-/TK^- Gene Deleted
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摘要 为了研制猪伪狂犬病病毒三基因缺失疫苗,本试验在前期工作的基础上,将TK基因缺失的质粒pBTK与gI-/gE-PRV SA738病毒基因组共转染BHK21细胞,通过蚀斑法进行筛选。用鉴定成功的重组病毒制备活疫苗,分别接种新生仔猪及妊娠母猪,观察仔猪、妊娠母猪产仔数及仔猪健康情况,并进行抗体中和试验和攻毒保护试验。同时与双基因缺失株gI-/gE-/PRV和PRV Bartha k61做对比,以生理盐水做对照。结果成功筛选到重组病毒,命名为gI-/gE-/TK-/PRV SA738,TCID50为5.75~6.125。重组病毒制备的活疫苗,对仔猪和妊娠母猪健康状况均无影响,安全性好,免疫保护率为100%(5/5),有效诱导了机体的保护性免疫应答。结果表明,gI-/gE-/TK-PRV SA738突变株适合作为疫苗毒株使用。 In order to study three-gene deleted vaccine strain of Pseudorabies virus,the plasmid pBTK- and gI-/gE-/PRV genome were co-transfected into BHK-21 by plaque method for screening. The recombinant virus was inoculated in newborn piglets and pregnant sows,then the antibody neutralization test and attack drug protection test were carried out. While the gI /gE-/PRV group and PRV Bartha k61 group were contrasted and saline as control. The results showed that the recombinant virus named by gI-/gE-/TK- / PRV SA738 with TCIDs0 5.75-6. 125 was successfully screened. It was safe for newborn piglets and pregnant sows,inducing protective immune response efficiently and with a strong and lasting antibody level, with immune protection 100% （5/5）. The results indicated that the gI-/gE-/TK- PRV SA738 was a suitable vaccine strain.
作者 吕素芳 郭广君 魏凤 王文秀 董炳梅 毕研丽 苗立中 沈志强
机构地区 山东省滨州畜牧兽医研究院 山东省畜禽蜂胶疫苗开发推广中心 山东绿都生物科技有限公司 山东省滨州畜牧兽医研究院山东省畜禽蜂胶疫苗开发推广中心
出处 《动物医学进展》 CSCD 北大核心 2014年第4期1-5,共5页 Progress In Veterinary Medicine
基金 山东省自然科学基金项目(ZR2012CQ012) 滨州市应用技术研究与开发专项(200706) 山东省技术创新项目(201220916006)
关键词 伪狂犬病病毒 gI- gE-基因 gI- gE- TK-基因 基因缺失 安全性 免疫效力 Pseudorabies virus gI-/gE- genes gI-/gE-/TK genes gene deletion safety immunity efficlency
分类号 S852.659.1 [农业科学—基础兽医学]
  • 相关文献

参考文献10

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二级参考文献17

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同被引文献127

引证文献19

二级引证文献134

动物医学进展
2014年 第4期

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