摘要
为了检测味觉受体偶联G蛋白Gγ13和α-gustducin基因在雌性生殖系统的表达,探讨2种G蛋白在雌性生殖机能中可能发挥的作用及其在生殖医学方面的潜在应用。用RT-PCR和超敏ABC免疫组化方法,检测Gγ13和α-gustducin在6周龄间情期小鼠卵巢、子宫、输卵管组织中的基因表达和蛋白分布。结果表明,在小鼠雌性生殖系统中均发现Gγ13和α-gustducin基因的转录子,免疫组化研究进一步证实了Gγ13和α-gustducin确实在小鼠卵巢颗粒黄体细胞、卵母细胞、子宫外膜、子宫内膜腺体、子宫肌层、输卵管浆膜和输卵管黏膜处存在阳性分布。本试验表明味觉受体偶联G蛋白Gγ13和α-gustducin在雌性小鼠生殖系统中广泛分布,预示着雌性生殖系统也可能对味觉物质进行感知,为进一步研究味觉受体在雌性生理机能中可能发挥的调控功能奠定初步的研究基础,同时也为味觉的细胞生物学研究提供新方向。
The aim of study was to detect gene expression of taste receptor G protein coupling Gγ13 and α- gustducin in the female reproductive system,and discuss the potential role that two G proteins play in regu- lating the female reproduction. RT-PCR and ABC staining was used in the current experiments. The ovary, uterus and oviduct from 6 week aged mice in dioestrus were used in the current experiments. We investigated the expression of Gγ13 and α-gustducin, the histological distribution of two proteins. The results revealed the presence of Gγ13 and α-gustducin mRNA in female reproductive system. Immunohistochemistry analysis showed that the positive signals were also observed in ovarian granular lutein cells, oocyte, perime- trium,uterine glands,myometrium,tunica serosa of oviduct and mucosa of oviduct. In short,the current results showed the wide distribution of Gγ13 and α-gustducin in the female reproductive system, indicated that female reproductive system can ＂sense＂ the tastant. This study provides a support for further work for taste receptors on how to affect the physiological function of the female reproductive system, and open a new window for the study of chemical senses.
出处
《动物医学进展》
CSCD
北大核心
2014年第4期39-46,共8页
Progress In Veterinary Medicine
基金
国家自然科学基金项目(81170756/H0713)
