酿酒白葡萄品种的SSR分析与鉴定

SSR Analysis and Identification of Grape Cultivars for Making White Wine

利用筛选出的14对引物对13个酿酒白葡萄品种进行SSR分析与鉴定，共扩增出312个条带，多态性条带共283个（多态性条带率为90．71％），特异性条带共78个（特异性条带率为25．00％）；13个品种均有特征性引物，二号白香和雷司令有5个特征性引物；VrZAG79条带总数、多态性条带和特异性条带均为最多（是7个品种的特征性引物），且所有品种的遗传差异性较大；14对引物的鉴别效率为15．38％（VVMD5）～100％（VrZAG62，VVIb66），即VrZAG62，VVIb66可分别鉴定该13个酿酒白葡萄品种且每个引物的鉴别效率差异很大。

Fourteen SSR primers screened were adopted to identify 13 grape cultivars used to make white wine. Three hundreds and twelve bands were altogether obtained from the amplification, including 283 polymorphic bands （90. 71%） and 78 specific bands （25.00%）. All the 13 cultivars maintained specific primers. For the cultivars Yarly Madeleine and Riesling, there were five specific primers each. Among 14 primers, VrZAG79, which was the specific primer for seven cultivars, owned more total bands, polymor- phic bands and specific bands, indicating large difference among different cultivars. The identification effi- ciency of 13 cultivars ranged between 15.38% （VVMDS）-100% （VrZAG62, VVIb66）. Large difference was shown among different cultivars and two primers （VrZAG62, VVIb66） could identify all the cultivars respectively.