洗毛用头状丝孢酵母产脂肪酶发酵条件研究

Optimization of Protease Production Fermentation Conditions by Trichosporon Capitatum

为了提高洗净毛质量,探索环境友好的洗毛方法,对头状丝孢酵母产脂肪酶的发酵条件进行研究,采用逐因子试验筛选出菌株Trichosporon capitatum的最佳碳源和氮源分别为可溶性淀粉、黄豆粉和(NH4)2SO4。其最优发酵条件为:培养基初始pH 6.5、接种量2%、250 mL三角瓶中最佳装液量30 mL、发酵温度28℃、发酵产酶转速160 r/min、诱导剂橄榄油5 g/L、表面活性剂吐温-80 1.5 g/L。

In order to improve the quality of wool washing and get the environmentally friendly wool washing method, tfichosporon capitatum fermentation conditions of lipase production were studied. Soluble starch, soybean meal and （ NH4 ） 2 SO4 were screened out as the most suitable carbon and nitrogen sources using single factor experiment. The optimal fermentation concentrations were determined, they were： initial pH 6.5, the best fluid volume in 250 mL conical flask 30 mL, fermentation temperature 28 ℃, rotating speed 160r/min, olive oil 5 g/L, Tween- 80 1.5 g/L.