不同形态的果糖对SD大鼠炎症因子及内脏组织的影响

Effects of Free and Bound Fructose on Inflammatory Factors and Viscera Histopathology in Sprague-Dawley Rat

探讨长期喂养游离态果糖和结合态果糖对大鼠炎症因子及内脏组织病理的影响。雄性SD大鼠40只,随机分为4个实验组及1个对照组,经过11 w喂养后,处死解剖,ELISA法检测血清及肝脏单核细胞趋化蛋白-1(MCP-1)与肿瘤坏死因子-α(TNF-α)的质量分数,苏木素-伊红(HE)染色法观察肝脏、胰脏组织病理学变化。结果显示,与对照组相比,含果糖实验组大鼠血清及肝脏MCP-1与TNF-α的质量分数均高于对照,其中游离态果糖组(F/G)血清MCP-1与TNF-α质量分数显著低于结合态果糖组(SU)(P<0.05)。同时,肝脏组织HE染色显示含果糖实验组均有不同程度的脂肪沉积,SU和F/ST(果糖/淀粉组)组脂质沉积现象比F/G组严重;各组大鼠胰脏组织差异不明显,但SU与F/ST组胰岛有轻微的颗粒样变性。因此推断:长期摄入不同形态高果糖均可能引起肝脏炎症及脂质沉积,结合态果糖可能会比游离态果糖引起更为严重的组织损伤。

Long-term effects of free and bound fructose on inflammatory factors and viscera histopathology in Sprague-Dawley rat were investigated.Male rats were randomized into four experimental groups and a control group.Each group was fed a diet containing 68％ of total calories as different composition of carbohydrate for 11 weeks.At the end of the experiment,rats were sacrificed by anesthesia and were autopsied.Serum monocyte chemoattractant protein-1 （MCP-1） and tumor necrosis factor-α（TNF-α） were detected by ELISA method.The pathological changes of liver and pancreas were directed with hematoxylin ＆ eosin（H＆E） staining.The results showed that the levels of serum and liver TNF-α and MCP-1 in fructose-fed rats were higher than the control.Moreover,the bound fructose（i.e.sucrose） showed higher serum MCP-1 than the free fructose（F/G） （P＜0.05）.Meanwhile,all of the three fructose containing diets induced various levels of hepatic steatosis,especially the SU and F/ST diet showed more severe fatty liver than F/G.The impairment of pancreas was unobvious,only the SU and F/ST groups showed slightly granular degeneration.It was suggested that long-term intake of high dose of fructose maybe induce inflammation and liver impairment,and bound fructose might produce more severe damage to health than the free fructose.