摘要
目的 鉴定2012年上海市虹口区麻疹流行株基因型,了解2000-2012年上海市麻疹流行株基因特性.方法 采集疑似麻疹病例咽拭子标本,使用Vero-SLAM细胞分离麻疹病毒,使用RT-PCR扩增分离培养阳性病毒N基因羧基末端450 bp片段并测序,与GenBank中的部分流行株构建系统进化树,分析麻疹病毒基因型,同时进行流行病学调查.结果 34份咽拭子标本中分离到麻疹病毒7株,进化树分析显示,6株为H1基因型,1株为D8基因型.流行病学调查显示,D8基因型为上海自2000年开展病毒监测以来的首次外来输入病毒.结论 2012年虹口区麻疹分离株与历年上海市麻疹流行株属于同一基因型,Hla亚型为优势流行株,首次出现D8基因型,为外来输入病毒.
Objective To ascertain the genotype of measles viruses isolated in 2012 and genetic characterization of measles viruses in Hongkou district of Shanghai during 2000-2012.Methods Measles virus was isolated from throat swab specimens collected from suspected measles cases and 450 bp fragment of C terminus on nucleprotein （N） gene was amplified by RT-PCR.Sequence analysis was conducted to ascertain the genotype and to compare the difference of nucleotide with other measles virus strain announced by GenBank during 2000-2012.Measles virus genotype was analyzed.Epidemiological investigation was conducted.Results Phylogenetic analysis showed that 7 measles virus samples were isolated from 34 throat swab specimens with 6 of them belonged to H1 genotype,1 belonged to D8 genotype of H1 genotype.H1a appeared the main part of Shanghai measles virus.Epidemiological survey showed that D8 was an imported case,also the first case detected since 2000.Conclusion The genotype distribution of measles virus in Hongkou was identified the same as elsewhere in Shanghai.D8 was an imported case,detected for the first time since 2000.The results suggested that viral gene sequencing and genotyping should be regularly conducted at the measles laboratories in Shanghai to strengthen the networking monitoring program of the disease.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2014年第4期429-432,共4页
Chinese Journal of Epidemiology
基金
上海市卫生局科研计划课题（20114067）
上海市公共卫生优秀学科带头人培养计划（GWDTR201225）
