TRPV1及TGF-β2的检测对儿童UACS诊断意义的探讨

The value of TRPV1 and TGF-β2 tests for the diagnose of UACS in children

目的探讨对瞬时受体电位香草酸受体1(TRPV1)及转化生长因子β2(TGF-β2)的检测在儿童UACS中的诊断价值。方法选择32例于我院行腺样体手术的腺样体肥大患儿,其中上气道咳嗽综合征(UACS)组16例,对照组16例。所有患儿均于术前采血进行ELISA检测,术中取腺样体标本进行免疫组化试验。结果 UACS组与对照组相比,用ELISA法和免疫组化法进行检测,TRPV1及TGF-β2的检测值为(14.84±4.38)pg/ml和(159.02±2.17)pg/ml,较对照组[(4.90±5.64)pg/ml和(101.49±1.70)pg/ml]明显升高,差异有统计学意义(P<0.05)。ELISA与免疫组化检测结果之间无统计学意义的相关性。免疫组化的敏感性较高,阴性预测值较高,ELISA的特异性较高。结论 TRPV1及TGF-β2的ELISA及免疫组化检测对UACS有辅助诊断价值。此两项诊断试验具有一定程度的互补性。

Objective The aim of the study was to investigate upper airway cough syndrome （UACS） in children and to determine alternative methods to explore the relationships among transient receptor potential vanniloid 1 （TRPV1） and transforming growth factor-β2 （TGF-β2） and UACS. Methods Thirty-two children with adenoid hypertrophy were included in the study. All children were tested for TRPV1 and TGF-β2 with Enzyme-linked immunosorbent assay（ELISA） and immunohistochemical （IHC）studies. Results The levels of TRPV1 and TGF-β2 were significantly increased in the UACS cases. There was no statistical correlation between ELISA and IHC testing. IHC had higher sensitivity and lower negative likelihood ratio, while ELISA had higher specificity. Conclusion The detections of TRPV1 and TGF-β2 from serum and adenoid body specimens are valuable for the auxiliary diagnosis of UACS. The two diagnostic tests are partially complementary.