雏菊叶龙胆促进大鼠坐骨神经功能恢复及机制研究

Experimental study of bellidifolin enhancing rehabilitation of injuried sciatic nerve

目的 观察中药雏菊叶龙胆（Bellidifolin）对坐骨神经损伤后的大鼠是否有促进神经功能恢复的作用,并探讨其机制中是否有睫状神经生长因子的参与.方法 雄性Wistar大鼠225只,制备坐骨神经损伤模型,大鼠右侧坐骨神经离断,手术显微镜下缝合,取左侧为空白对照.造模成功后,大鼠随机分为对照组、雏菊叶龙胆25 mg组、50 mg组、75 mg组和甲钴胺组共5组,每组45只.对照组给予生理盐水腹腔注射,其余各组于手术后每天给予相应剂量的雏菊叶龙胆及甲钴胺腹腔注射,1,3及5周后进行足踪分析,计算坐骨神经功能指数（Sciatic Functional Index,SFl）、小腿三头肌湿重,免疫组化检测坐骨神经中组织睫状神经营养因子的变化（Ciliary Neurotrophic Factor,CNTF）.结果 3周时SFI检测结果显示,对照组、甲钴胺组及雏菊叶龙胆25 mg、50 mg、75 mg组分别为：-84.35±4.87,-45.20±2.3,-70.42±4.2,-57.73±3.46和-64.38±4.38,其中对照组与各组相比差异有统计学意义（P＜0.05）,甲钴胺组与雏菊叶龙胆各组相比结果差异有统计学意义（P＜0.05）,雏菊叶龙胆组各组间相比,25 mg、75 mg与50 mg组相比差异有统计学意义（P＜0.05）.小腿三头肌湿重恢复率比较,雏菊叶龙胆各组与对照组、甲钴胺组相比差异有统计学意义（P＜0.05）,其中雏菊叶龙胆组中25 mg.75 mg组与50 mg组相比,差异有统计学意义（P＜0.05）.3周时各组进行CNTF免疫组化比较,可见雏菊叶龙胆组25 mg、70 mg组与50 mg组相比差异有统计学意义（Jp＜0.05）.结论 雏菊叶龙胆可以促进损伤坐骨神经的功能恢复,50 mg作用最明显,神经组织内CNTF因子发挥重要作用.

Objective To study whether bellidifolin has rehabilitation effect on injured sciatic nerve rats, and whether cilial7 neurotrophic factor （CNTF） is involved in this mechanism. Methods 225 male wistar rats were made to be sciatic nerve injured models, with right sciatic nerve being cut and sewed under microscopy, left sciatic being sham. Rats were randomly divided into control group, bellidifolin 25 rag, 50 rag,75 mg groups and mecobalamin group,with 45 rats in each group.Rats in control group were just injected sodium chloride, others were intra-peritonealiy injected different doses of bellidifolin and mecobalamin after operation. Results Three weeks af- ter operation, SFI of control group, mecobalaming group, bellidifolin 25 rag,50 nag and 75 mg group were -84.35± 4.87 ,-45.20±2.30,-70.42±4.21 ,-57.73±3.46 and -64.38±4.38 respectively.Compared with control group,oth- ers showed significant differences （P〈 0. 05 ） . There were statistically differences between bellidifolin groups and mecobalaming group（P〈0.05）.Within bellidifolin groups,50 mg group showed difference compared with 25 mg and 75 mg groups（P= 0.031 ）.TSW results also showed differences among bellidifolin groups,control group and meco- balaming group.There were statistical differences among bellidifolin groups（P〈0.05）. Each groups with immunohis- toehemistry analysis, CNTF expression showed statistically differences among bellidifo[in 50 mg group and 25 rag, 70 mg groups. Bellidifolin 50 mg group was higher than others（P〈0.05）. Conclusion Bellidifolin can promote the recovery of injured sciatic nerve, especially the concentration of 50 mg bellidifolin, and CNTF is involved in the re- hablitation process.