消痹膏镇痛抗炎作用的实验研究

Experimental Research on Analgesic and Anti-inflammatory Effects of “Xiaobi Ointment”

目的:评价中药复方外敷剂消痹膏的镇痛、抗炎作用。方法:将ICR小鼠(或SD大鼠)随机分为5组:阴性对照组(黄凡士林)、阳性对照组(扶他林乳胶剂)及消痹膏低、中、高剂量组,分别于腹部脱毛处外敷相应药物。采用热板法、醋酸法致小鼠疼痛模型,外涂二甲苯致小鼠急性炎症模型,注射角叉菜胶致大鼠慢性炎症模型,考察各组的镇痛、抗炎作用。结果:镇痛实验显示,消痹膏高剂量组可明显提高热板法致小鼠疼痛痛阈值,且优于阳性对照组,但差异无统计学意义;消痹膏各剂量组及阳性对照组均可明显抑制醋酸法致小鼠疼痛反应(P<0.01),且呈一定的量效关系。抗炎实验显示,消痹膏各剂量组及阳性对照组均可显著抑制二甲苯致小鼠耳肿胀度、抑制角叉菜胶致小鼠足肿胀度(P<0.01,P<0.05)。结论:消痹膏有明显的镇痛、抗炎作用。

Objective： To evaluate the analgesic and anti-inflammatory effects of ＂Xiaobi Ointment＂. Methods： ICR mice （or SD rats ） were randomly divided into 5 groups： negative control group （Yellow Vaseline ）, positive control group （Diclofenac Diethylamine Emulgel） and ＂Xiaobi Ointment＂ groups （low, medium and high dose）. The rats were treated with corresponding measures externally. The models were established by corresponding methods to evaluate the analgesic and anti- inflammatory effects of ＂Xiaobi Ointment＂. Results： Analgesic experiments showed that ＂Xiaobi Ointment＂ high dose group could obviously elevate the pain threshold by hot plate test, and without statistical significance between high dose group and positive control group ; ＂ Xiaobi Ointment＂ groups and positive control group could significantly inhibit the pain reaction induced by acetic acid method （ P 〈 0.01 ）, with dose-effect relationship. The anti-inflammatory experiments showed that ＂Xiaobi Ointment＂ groups and positive control group could restrain ear swelling degree caused by xylene and foot swelling degree caused by Carrageen glue （P〈0.01 or P〈0.05）. Conclusion： ＂Xiaobi Ointment＂ has analgesic and anti- inflammatory effects.