甘草药材HPLC指纹图谱研究

Research on HPLC Fingerprint Spectrum of Radix Glycyrrhizae

目曲：建立甘草药材HPLC指纹图谱，并尝试将其应用到质量评价中。方法：采用RP—HPLC（DAD）法，用AgilentExtend—C18色谱柱（250mm×4．6mm，5μm）和Extend-C18保护柱（20mm×4．6mm，5μm）。以乙腈（A）-0．026％磷酸水溶液（B）为流动相。采用梯度洗脱：0～20min，A为20％～25％；20—30min，A为25％～33％；30～55min，A为33％～50％；55—65min，A为50％-60％；65～80min，A为60％。检测波长为280nm。结果：对29批甘草药材进行测定，标定了29个共有峰，且样品基本上被分成了三类。结论：建立了高效液相指纹图谱（RP—HPLC），能有效地体现出中药成分的复杂性；对29批甘草药材进行的相似度评价，反应出一定的差异性，推测这三类甘草的差异可能为种的不同所致。

Objective： To construct HPLC Fingerprint Spectrum of Radix Glycyrrhizae and apply it into quality assessment. Methods： By the method of HPLC-DAD, the analysis was performed on an Agilent Extend-C18 column and Extend-C18. The mobile phase was consisted of acetonitrile （A） and aqueous H3 PO4 （B）. The gradient elution of 20% - 25 % A at 0-20 rain ; 25%-33% A at 20-30 min; 33%-50% A at 30-55 min; 50%-60% A at 55-65 min; 60% A at 65-80 min. We detected the wave length was 280 nm. Results： Twenty-nine batches of Radix Glycyrrhizae were determined and 29 common peaks were demonstrated. The samples were clarified into 3 categories. Conclusion： The RP-HPLC was established to reflect the complexity of material medica; after evaluating the similarity of 29 batches of Radix Glycyrrhizae, we found there was significant difference among them, thus we speculate about these 3 categories of Radix Glycyrrhizae are different species.